@article{c620c12ff3e64420a3dbde3e35b6075a,
title = "IgE-mediated regulation of IL-10 and type I IFN enhances rhinovirus-induced Th2 differentiation by primary human monocytes",
abstract = "Rhinovirus (RV) infections are linked to the development and exacerbation of allergic diseases including allergic asthma. IgE, another contributor to atopic disease pathogenesis, has been shown to regulate DC antiviral functions and influence T cell priming by monocytes. We previously demonstrated that IgE-mediated stimulation of monocytes alters multiple cellular functions including cytokine secretion, phagocytosis, and influenza-induced Th1 development. In this study, we investigate the effects of IgE-mediated stimulation on monocyte-driven, RV-induced T cell development utilizing primary human monocyte-T cell co-cultures. We demonstrate that IgE crosslinking of RV-exposed monocytes enhances monocyte-driven Th2 differentiation. This increase in RV-induced Th2 development was regulated by IgE-mediated inhibition of virus-induced type I IFN and induction of IL-10. These findings suggest an additional mechanism by which two clinically significant risk factors for allergic disease exacerbations—IgE-mediated stimulation and rhinovirus infection—may synergistically promote Th2 differentiation and allergic inflammation.",
keywords = "IL-10, IgE, Th2, interferon, monocyte, rhinovirus",
author = "Rowe, {Regina K.} and Pyle, {David M.} and Farrar, {J. David} and Gill, {Michelle A.}",
note = "Funding Information: We thank the participants, whose participation made our study possible. We thank Jim Gern, M.D. and Yury Bochkov, Ph.D. (U Wisconsin) for providing highly purified RV-16. We acknowledge Yaging Gao and Robert Maples for assistance with PBMC irradiation, members of the UTSW Farrar lab for helpful discussions, and Angela Mobley and the UTSW Immunology Flow Cytometry core staff for assistance with flow cytometry analysis. Figure 5 was created with Biorender. We thank the following funding sources: NIH NIAID, R01-AI098077 (MAG), Children's Clinical Research Advisory Council (MAG), William A. and Joyce M. Sellars Distinguished Chair in Allergy and Immunology (MAG), NIH NHLBI, T32-HL098040 (RKR), Pediatric Infectious Disease Society/Astra-Zeneca Fellow Award in Respiratory Virus Research (RKR), NIH NRSA, T32-AI005284 (DMP), NIH NIGMS, T32-GM008014 (DMP). Funding Information: We thank the participants, whose participation made our study possible. We thank Jim Gern, M.D. and Yury Bochkov, Ph.D. (U Wisconsin) for providing highly purified RV‐16. We acknowledge Yaging Gao and Robert Maples for assistance with PBMC irradiation, members of the UTSW Farrar lab for helpful discussions, and Angela Mobley and the UTSW Immunology Flow Cytometry core staff for assistance with flow cytometry analysis. Figure 5 was created with Biorender. We thank the following funding sources: NIH NIAID, R01‐AI098077 (MAG), Children's Clinical Research Advisory Council (MAG), William A. and Joyce M. Sellars Distinguished Chair in Allergy and Immunology (MAG), NIH NHLBI, T32‐HL098040 (RKR), Pediatric Infectious Disease Society/Astra‐Zeneca Fellow Award in Respiratory Virus Research (RKR), NIH NRSA, T32‐AI005284 (DMP), NIH NIGMS, T32‐GM008014 (DMP). Publisher Copyright: {\textcopyright} 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2020",
month = oct,
day = "1",
doi = "10.1002/eji.201948396",
language = "English (US)",
volume = "50",
pages = "1550--1559",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "10",
}