Identification, structural determination, and biological activity of bovine and canine calcitonin receptor-stimulating peptides

Takeshi Katafuchi, Kazumasa Hamano, Naoto Minamino

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


We have recently identified in porcine brain a series of new peptides, designated calcitonin receptor-stimulating peptide-1 (CRSP-1), CRSP-2, and CRSP-3, but failed to find their counterparts in humans and rodents by either database searching or experimental cross-hybridization. In this study, we isolated cDNAs encoding precursors of bovine CRSP-1, canine CRSP-1, and canine CRSP-2 from their thyroid cDNA libraries. Although the deduced mature amino acid sequences of bovine and canine CRSP-1s and canine CRSP-2 showed identity with their respective porcine CRSP counterparts, none of them had a C-terminal amide structure. In LLC-PK1 cells endogenously expressing the calcitonin (CT) receptor, bovine and canine CRSP-1s enhanced the cAMP production, while canine CRSP-2 did not stimulate it at all. Equine CGRP-I had a high identity in its amino acid sequence with porcine CRSP-1 and stimulated LLC-PK1 cells at a potency comparable to that of porcine CT. None of these CRSPs or equine CGRP-I stimulated the CT-like receptor, even in the presence of receptor activity-modifying proteins. These results demonstrate that CRSP-1, a new class of biologically active peptide, is present in animals evolutionarily close to pigs and induces its activity through the calcitonin receptor, suggesting a wide existence and common properties of this peptide in mammals.

Original languageEnglish (US)
Pages (from-to)74-79
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number1
StatePublished - Jan 2 2004


  • Calcitonin
  • Calcitonin gene-related peptide
  • Calcitonin receptor
  • Calcitonin receptor-stimulating peptide
  • Calcitonin-like receptor
  • Receptor activity-modifying protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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