TY - JOUR
T1 - Identification of the transcriptional targets of FOXP2, a gene linked to speech and language, in developing human brain
AU - Spiteri, Elizabeth
AU - Konopka, Genevieve
AU - Coppola, Giovanni
AU - Bomar, Jamee
AU - Oldham, Michael
AU - Ou, Jing
AU - Vernes, Sonja C.
AU - Fisher, Simon E.
AU - Ren, Bing
AU - Geschwind, Daniel H.
N1 - Funding Information:
This work is submitted in grateful memory of Zheng Luo, who performed the pilot ChIP-chip experiments in the Geschwind laboratory. His technical skills were essential to establishing this technique, and his presence is very much missed. We thank the NICHD Brain and Tissue Bank for Developmental Disorders, under contracts N01-HD-4-3368 and N01-HD-4-3383, for providing human fetal brain tissue. We also thank Eric Wexler, M.D., Ph.D., and other members of the Geschwind laboratory for helpful discussions, as well as an anonymous reviewer whose insightful comments brought several interesting targets to our attention and highlighted some of the key evolutionary context. This research was supported by grants R21MH075028 (to D.H.G.), T32GM008243 (to E.S.), and T32HD007032 (to G.K.).
PY - 2007
Y1 - 2007
N2 - Mutations in FOXP2, a member of the forkhead family of transcription factor genes, are the only known cause of developmental speech and language disorders in humans. To date, there are no known targets of human FOXP2 in the nervous system. The identification of FOXP2 targets in the developing human brain, therefore, provides a unique tool with which to explore the development of human language and speech. Here, we define FOXP2 targets in human basal ganglia (BG) and inferior frontal cortex (IFC) by use of chromatin immunoprecipitation followed by microarray analysis (ChIP-chip) and validate the functional regulation of targets in vitro. ChIP-chip identified 285 FOXP2 targets in fetal human brain; statistically significant overlap of targets in BG and IFC indicates a core set of 34 transcriptional targets of FOXP2. We identified targets specific to IFC or BG that were not observed in lung, suggesting important regional and tissue differences in FOXP2 activity. Many target genes are known to play critical roles in specific aspects of central nervous system patterning or development, such as neurite outgrowth, as well as plasticity. Subsets of the FOXP2 transcriptional targets are either under positive selection in humans or differentially expressed between human and chimpanzee brain. This is the first ChIP-chip study to use human brain tissue, making the FOXP2-target genes identified in these studies important to understanding the pathways regulating speech and language in the developing human brain. These data provide the first insight into the functional network of genes directly regulated by FOXP2 in human brain and by evolutionary comparisons, highlighting genes likely to be involved in the development of human higher-order cognitive processes.
AB - Mutations in FOXP2, a member of the forkhead family of transcription factor genes, are the only known cause of developmental speech and language disorders in humans. To date, there are no known targets of human FOXP2 in the nervous system. The identification of FOXP2 targets in the developing human brain, therefore, provides a unique tool with which to explore the development of human language and speech. Here, we define FOXP2 targets in human basal ganglia (BG) and inferior frontal cortex (IFC) by use of chromatin immunoprecipitation followed by microarray analysis (ChIP-chip) and validate the functional regulation of targets in vitro. ChIP-chip identified 285 FOXP2 targets in fetal human brain; statistically significant overlap of targets in BG and IFC indicates a core set of 34 transcriptional targets of FOXP2. We identified targets specific to IFC or BG that were not observed in lung, suggesting important regional and tissue differences in FOXP2 activity. Many target genes are known to play critical roles in specific aspects of central nervous system patterning or development, such as neurite outgrowth, as well as plasticity. Subsets of the FOXP2 transcriptional targets are either under positive selection in humans or differentially expressed between human and chimpanzee brain. This is the first ChIP-chip study to use human brain tissue, making the FOXP2-target genes identified in these studies important to understanding the pathways regulating speech and language in the developing human brain. These data provide the first insight into the functional network of genes directly regulated by FOXP2 in human brain and by evolutionary comparisons, highlighting genes likely to be involved in the development of human higher-order cognitive processes.
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U2 - 10.1086/522237
DO - 10.1086/522237
M3 - Article
C2 - 17999357
AN - SCOPUS:36749050396
SN - 0002-9297
VL - 81
SP - 1144
EP - 1157
JO - American Journal of Human Genetics
JF - American Journal of Human Genetics
IS - 6
ER -