Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus

Tong Jin Zhao; Yang Liu; Yong Bin Yan; Feng Feng; Wei Qun Liu; Hai Meng Zhou

doi:10.1016/j.febslet.2007.05.067

Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus

Tong Jin Zhao, Yang Liu, Yong Bin Yan, Feng Feng, Wei Qun Liu, Hai Meng Zhou

Molecular Genetics

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

We have previously identified two homologous groups of BnDREBs in Brassica napus, the trans-active BnDREBI and the trans-inactive BnDREBII, which provided an ideal system to study the trans-activation of DREB1/CBF. Deletion analysis indicated that the two additional regions in BnDREBI contributed little to the transcriptional activity. Domain swapping analysis indicated that all the domains contributed to the activity of BnDREBI, including the ERF/AP2 DNA binding domain. Through site-directed mutagenesis, we identified nine residues that were involved in the activity of BnDREBI, among which six residues are specific to BnDREBI, and three are common to DREB1A.

Original language	English (US)
Pages (from-to)	3044-3050
Number of pages	7
Journal	FEBS Letters
Volume	581
Issue number	16
DOIs	https://doi.org/10.1016/j.febslet.2007.05.067
State	Published - Jun 26 2007

Keywords

Brassica napus
DRE
DREB1/CBF
Trans-activation
Transcriptional factors

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2007.05.067

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title = "Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus",

abstract = "We have previously identified two homologous groups of BnDREBs in Brassica napus, the trans-active BnDREBI and the trans-inactive BnDREBII, which provided an ideal system to study the trans-activation of DREB1/CBF. Deletion analysis indicated that the two additional regions in BnDREBI contributed little to the transcriptional activity. Domain swapping analysis indicated that all the domains contributed to the activity of BnDREBI, including the ERF/AP2 DNA binding domain. Through site-directed mutagenesis, we identified nine residues that were involved in the activity of BnDREBI, among which six residues are specific to BnDREBI, and three are common to DREB1A.",

keywords = "Brassica napus, DRE, DREB1/CBF, Trans-activation, Transcriptional factors",

author = "Zhao, {Tong Jin} and Yang Liu and Yan, {Yong Bin} and Feng Feng and Liu, {Wei Qun} and Zhou, {Hai Meng}",

note = "Funding Information: This work was supported by funds from the National Key Science and Technology Item of China (2002AA224091 and 2005AA224090), the National Key Basic Research Specific Foundation of China (G 1999075607), the Natural Science Foundation of China (30221003, 30429001 and 60401009) and Funds from Jiaxing, Zhejiang.",

year = "2007",

month = jun,

day = "26",

doi = "10.1016/j.febslet.2007.05.067",

language = "English (US)",

volume = "581",

pages = "3044--3050",

journal = "FEBS Letters",

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number = "16",

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T1 - Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus

AU - Zhao, Tong Jin

AU - Liu, Yang

AU - Yan, Yong Bin

AU - Feng, Feng

AU - Liu, Wei Qun

AU - Zhou, Hai Meng

N1 - Funding Information: This work was supported by funds from the National Key Science and Technology Item of China (2002AA224091 and 2005AA224090), the National Key Basic Research Specific Foundation of China (G 1999075607), the Natural Science Foundation of China (30221003, 30429001 and 60401009) and Funds from Jiaxing, Zhejiang.

PY - 2007/6/26

Y1 - 2007/6/26

N2 - We have previously identified two homologous groups of BnDREBs in Brassica napus, the trans-active BnDREBI and the trans-inactive BnDREBII, which provided an ideal system to study the trans-activation of DREB1/CBF. Deletion analysis indicated that the two additional regions in BnDREBI contributed little to the transcriptional activity. Domain swapping analysis indicated that all the domains contributed to the activity of BnDREBI, including the ERF/AP2 DNA binding domain. Through site-directed mutagenesis, we identified nine residues that were involved in the activity of BnDREBI, among which six residues are specific to BnDREBI, and three are common to DREB1A.

AB - We have previously identified two homologous groups of BnDREBs in Brassica napus, the trans-active BnDREBI and the trans-inactive BnDREBII, which provided an ideal system to study the trans-activation of DREB1/CBF. Deletion analysis indicated that the two additional regions in BnDREBI contributed little to the transcriptional activity. Domain swapping analysis indicated that all the domains contributed to the activity of BnDREBI, including the ERF/AP2 DNA binding domain. Through site-directed mutagenesis, we identified nine residues that were involved in the activity of BnDREBI, among which six residues are specific to BnDREBI, and three are common to DREB1A.

KW - Brassica napus

KW - DRE

KW - DREB1/CBF

KW - Trans-activation

KW - Transcriptional factors

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JF - FEBS Letters

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Identification of the amino acids crucial for the activities of drought responsive element binding factors (DREBs) of Brassica napus

Abstract

Keywords

ASJC Scopus subject areas

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