Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract

Z. Lu; W. Yang; Q. Cao; W. Tao; J. Hu; Z. Zhai

doi:10.1007/BF03187029

Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract

Z. Lu, W. Yang, Q. Cao, W. Tao, J. Hu, Z. Zhai

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2 Scopus citations

Abstract

When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with XAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that IXAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.

Original language	English (US)
Pages (from-to)	1449-1453
Number of pages	5
Journal	Chinese Science Bulletin
Volume	46
Issue number	17
DOIs	https://doi.org/10.1007/BF03187029
State	Published - 2001

Keywords

Apoptosis
Cell-free system
Cytochrome c
DNA Ladder
IXAD
XAD
Xenopus laevis

ASJC Scopus subject areas

General

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10.1007/BF03187029

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@article{39f975f98f514c9088ad9e7b3b6d06a5,

title = "Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract",

abstract = "When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with XAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that IXAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.",

keywords = "Apoptosis, Cell-free system, Cytochrome c, DNA Ladder, IXAD, XAD, Xenopus laevis",

author = "Z. Lu and W. Yang and Q. Cao and W. Tao and J. Hu and Z. Zhai",

note = "Funding Information: Acknowledgements We thank Prof. Wang Xiaodong with the North-west Medical Center of Texas for giving Rabbit anti-human DFF45 polyclonal antibody and pcDNA3 plasmid containing the full length DFF45 cDNA and the Zoology Institute of the CAS for providing materials. This work was supported by the National Basic Research Project (Grant No. G1999053905).",

year = "2001",

doi = "10.1007/BF03187029",

language = "English (US)",

volume = "46",

pages = "1449--1453",

journal = "Chinese Science Bulletin",

issn = "1001-6538",

publisher = "Springer Science + Business Media",

number = "17",

}

TY - JOUR

T1 - Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract

AU - Lu, Z.

AU - Yang, W.

AU - Cao, Q.

AU - Tao, W.

AU - Hu, J.

AU - Zhai, Z.

N1 - Funding Information: Acknowledgements We thank Prof. Wang Xiaodong with the North-west Medical Center of Texas for giving Rabbit anti-human DFF45 polyclonal antibody and pcDNA3 plasmid containing the full length DFF45 cDNA and the Zoology Institute of the CAS for providing materials. This work was supported by the National Basic Research Project (Grant No. G1999053905).

PY - 2001

Y1 - 2001

N2 - When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with XAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that IXAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.

AB - When added with cytochrome c, Xenopus laevis egg extract XS-150 can induce exogenous nuclei undergoing apoptosis. Apoptosis specific DNase XAD was activated during this process, and cut chromatin between nucleosome, leading to DNA Ladder in electrophoresis. Our results showed that an inhibitor of XAD, IXAD, exists abundantly in normal egg extract, its molecular weight is about 40 ku. Normally, IXAD exists either in the form of dimmer or in complex with XAD. It was degraded during apoptosis, releasing active XAD. The results of Western assay and cross-inhibition showed that IXAD was likely homologous to DFF45 in structure and function. At the same time, these results also indicated that the pathway in apoptosis was conserved in evolution.

KW - Apoptosis

KW - Cell-free system

KW - Cytochrome c

KW - DNA Ladder

KW - IXAD

KW - XAD

KW - Xenopus laevis

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U2 - 10.1007/BF03187029

DO - 10.1007/BF03187029

M3 - Article

AN - SCOPUS:0034834752

SN - 1001-6538

VL - 46

SP - 1449

EP - 1453

JO - Chinese Science Bulletin

JF - Chinese Science Bulletin

IS - 17

ER -

Identification of inhibitor of apoptosis specific DNase in Xenopus egg extract

Abstract

Keywords

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