Identification of chlorella T-1 ingredient which enhances DMPO-OH adduct formation in Fenton reaction

Chlorella T-1 is a micro algae which carries out highly active photosynthetic carbon fixation. For searching the ·OH scavenging molecules in this strain, the methanol extract of T-1 was fractionated by RP-HPLC and the fractions were analyzed for DMPO-OH formation by ESR after Fenton reaction. In the course of study, a single chlorella T-1 ingredient was found to enhance the formation of DMPO-OH rather than scavenge in the Fenton system. The ·OH enhancing chlorella component was further purified by chromatography and finally identified as lactic acid by NMR and LC-MS. From the analysis of the reaction conditions for DMPO-OH, it was found that lactic acid did not serve as direct source of ·OH but accelerated Fenton reaction.