Abstract
We report the cloning and characterization of a human cDNA predicted to encode a novel hydrophobic protein containing four transmembrane domains and a zinc metalloprotease motif, HEXXH, between the third and fourth transmembrane domains, and have named the molecule metalloprotease-related protein-1 (MPRP-1). The MPRP-1 gene was localized to chromosome 1-p32.3 by radiation hybrid mapping, and Northern blot analysis revealed expression in many organs, with strong expression in the heart, skeletal muscle, kidney and liver. Immunohistochemical analyisis showed that MPRP-1 was localized in the endoplasmic reticulum (ER), and not in the Golgi compartment. Fragments of DNA encoding a segment homologous to the HEXXH motif of MPRP-1 are widely found in bacteria, yeast, plants, and animals. These results suggest that the MPRP-1 may have highly conserved functions, such as in intracellular proteolytic processing in the ER.
Original language | English (US) |
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Pages (from-to) | 123-128 |
Number of pages | 6 |
Journal | DNA Research |
Volume | 10 |
Issue number | 3 |
DOIs | |
State | Published - 2003 |
Keywords
- Endoplasmic reticulum
- Membrane protein
- Metalloprotease
ASJC Scopus subject areas
- Molecular Biology
- Genetics