Abstract
A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which depended on Mg2+ and inhibited by Zn2+ . Two nucleases existing in egg extracts were revealed by in-gel nuclease assay. Further experiments showed that 27 ku nuclease which was different from other Ca2+/Mg2+-dependent nucleuses was a possible candidate involved in apoptosis.
Original language | English (US) |
---|---|
Pages (from-to) | 522-526 |
Number of pages | 5 |
Journal | Chinese Science Bulletin |
Volume | 43 |
Issue number | 6 |
DOIs | |
State | Published - 1998 |
Keywords
- Apoptosis
- Cell-free system
- Dnase
- Xenopus egg extracts
ASJC Scopus subject areas
- General