A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which depended on Mg2+ and inhibited by Zn2+ . Two nucleases existing in egg extracts were revealed by in-gel nuclease assay. Further experiments showed that 27 ku nuclease which was different from other Ca2+/Mg2+-dependent nucleuses was a possible candidate involved in apoptosis.
|Original language||English (US)|
|Number of pages||5|
|Journal||Chinese Science Bulletin|
|State||Published - 1998|
- Cell-free system
- Xenopus egg extracts
ASJC Scopus subject areas