Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis

Shan Zhu; Zhengfan Jiang; Bo Zhang; Zhigang Lu; Zhonghe Zhai

doi:10.1007/BF02883826

Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis

Shan Zhu, Zhengfan Jiang, Bo Zhang, Zhigang Lu, Zhonghe Zhai

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which depended on Mg²⁺ and inhibited by Zn²⁺ . Two nucleases existing in egg extracts were revealed by in-gel nuclease assay. Further experiments showed that 27 ku nuclease which was different from other Ca²⁺/Mg²⁺-dependent nucleuses was a possible candidate involved in apoptosis.

Original language	English (US)
Pages (from-to)	522-526
Number of pages	5
Journal	Chinese Science Bulletin
Volume	43
Issue number	6
DOIs	https://doi.org/10.1007/BF02883826
State	Published - 1998

Keywords

Apoptosis
Cell-free system
Dnase
Xenopus egg extracts

ASJC Scopus subject areas

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10.1007/BF02883826

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title = "Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis",

abstract = "A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which depended on Mg2+ and inhibited by Zn2+ . Two nucleases existing in egg extracts were revealed by in-gel nuclease assay. Further experiments showed that 27 ku nuclease which was different from other Ca2+/Mg2+-dependent nucleuses was a possible candidate involved in apoptosis.",

keywords = "Apoptosis, Cell-free system, Dnase, Xenopus egg extracts",

author = "Shan Zhu and Zhengfan Jiang and Bo Zhang and Zhigang Lu and Zhonghe Zhai",

note = "Funding Information: Acknowledgement This work was supported by the National Natural Science Foundation of China (Grant No. 19334030), and is a joint project in the framework of cooperation between Chinese Foundation of Natural Sciences and Russian Foundation of basic re- search. The photoluminescent experiments were finished in Vavilov Laboratory of Luminescence, P. N. Lebedev Physical Institute, Russion Academy of Sciences.",

year = "1998",

doi = "10.1007/BF02883826",

language = "English (US)",

volume = "43",

pages = "522--526",

journal = "Chinese Science Bulletin",

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publisher = "Springer Science + Business Media",

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TY - JOUR

T1 - Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis

AU - Zhu, Shan

AU - Jiang, Zhengfan

AU - Zhang, Bo

AU - Lu, Zhigang

AU - Zhai, Zhonghe

N1 - Funding Information: Acknowledgement This work was supported by the National Natural Science Foundation of China (Grant No. 19334030), and is a joint project in the framework of cooperation between Chinese Foundation of Natural Sciences and Russian Foundation of basic re- search. The photoluminescent experiments were finished in Vavilov Laboratory of Luminescence, P. N. Lebedev Physical Institute, Russion Academy of Sciences.

PY - 1998

Y1 - 1998

N2 - A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which depended on Mg2+ and inhibited by Zn2+ . Two nucleases existing in egg extracts were revealed by in-gel nuclease assay. Further experiments showed that 27 ku nuclease which was different from other Ca2+/Mg2+-dependent nucleuses was a possible candidate involved in apoptosis.

AB - A cell-free apoptosis system was established by adding dATP and cytochrome c to Xenopus laevis egg extracts S-150. Accompanied by an incubation process, an apoptosis-specific DNase was activated in egg extracts which depended on Mg2+ and inhibited by Zn2+ . Two nucleases existing in egg extracts were revealed by in-gel nuclease assay. Further experiments showed that 27 ku nuclease which was different from other Ca2+/Mg2+-dependent nucleuses was a possible candidate involved in apoptosis.

KW - Apoptosis

KW - Cell-free system

KW - Dnase

KW - Xenopus egg extracts

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U2 - 10.1007/BF02883826

DO - 10.1007/BF02883826

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SP - 522

EP - 526

JO - Chinese Science Bulletin

JF - Chinese Science Bulletin

IS - 6

ER -

Identification of a DNase activated in Xenopus egg extracts undergoing apoptosis

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Keywords

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