Identification and gene expression of bovine C-type lectin dectin-2

M. Bonkobara, M. Hoshino, H. Yagihara, K. Tamura, M. Isotani, Y. Tanaka, T. Washizu, K. Ariizumi

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


The C-type lectin receptor has been shown to recognize carbohydrate moieties of self and non-self antigens, thus serving as an innate immune receptor. Using bioinformatics and molecular cloning techniques, we isolated a bovine gene that encodes a polypeptide of 206 amino acids with structural features shared by mouse and human dectin-2, including a high homology with mouse dectin-2 (66%), a type II configuration, a short cytoplasmic domain without tyrosine-based signal motifs, a carbohydrate recognition domain, a putative N-glycosylation site, and an EPN motif involved in the Ca 2+-dependent binding of hexose carbohydrates. These results reveal this bovine gene to be a counterpart of mouse dectin-2. Moreover, the bovine dectin-2 gene showed heterogeneity in mRNA (the generation of alternatively spliced transcript) and segmentation into six exons, which are also observed in mouse dectin-2. Inconsistent with mouse dectin-2 mRNA, the bovine counterpart is abundantly expressed by Langerhans cells compared to macrophages; however, lymph nodes showed the highest expression level of bovine dectin-2, while spleen and lung showed the highest expression levels of mouse and human dectin-2. In cattle, dectin-2 expressed by dendritic cells may be clinically involved in the recognition of invading antigens in lymph nodes.

Original languageEnglish (US)
Pages (from-to)179-186
Number of pages8
JournalVeterinary Immunology and Immunopathology
Issue number1-2
StatePublished - Mar 15 2006


  • Bovine
  • C-type lectin receptor
  • Cloning
  • Dectin-2
  • Langerhans/dendritic cell

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)


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