Abstract
Here, we identified a novel peptide specifically targeting a human B cell lymphoma, Raji, through a conventional phage display method. The amino acid sequence, 'CTLPHLKMC' was obtained with the highest frequency from a nonapeptide-expressing phage library. The phage clone encoding CTLPHLKMC peptide sequence avidly bound to Raji cells compared with control phage clones. Furthermore, flow-cytometric analysis on the biotinylated synthetic CTLPHLKMC peptide demonstrated the high binding affinity to Raji cells in a dose-dependent manner whereas it has binding activity to neither human peripheral blood mononuclear cells including normal B cell derived from healthy donors nor other leukemia cells including THP-1, HL-60, Jurkat and IM-9. MALDI-TOF mass spectrometry following immunoprecipitation assay showed that a potential host receptor for the peptide is a variable region of human immunoglobulin heavy chain which would be a specific phenotypic marker of Raji. In conclusion, these results suggest that the peptide, 'CTLPHLKMC', is a specific ligand to a Raji cell.
Original language | English (US) |
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Pages (from-to) | 852-858 |
Number of pages | 7 |
Journal | International Immunopharmacology |
Volume | 8 |
Issue number | 6 |
DOIs | |
State | Published - Jun 2008 |
Externally published | Yes |
Keywords
- B cell lymphoma
- Phage display
- Raji
- Targeting peptide
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology
- Pharmacology