TY - JOUR
T1 - Identification and biological activity of dihydro-leukotriene B4
T2 - A prominent metabolite of leukotriene B4 in the human lung
AU - Kumlin, M.
AU - Falck, J R
AU - Raud, J.
AU - Harada, Y.
AU - Dahlén, S. E.
AU - Granström, E.
N1 - Funding Information:
We thank Ms. Eva Ohlson for excellent technical assistance, and the staff at the Department of Thoracic Surgery and Dr. Thure Bjiirck for great help with the collection of tissue specimens. We thank Drs. J. Rokach (Merck Frosst, Canada), R. Zipkin (Biomol Inc., U.S.A.) and D. Clissold (Cascade Ltd, U.K.) for kind supply of synthetic LTB4. This work was supported by the Swedish Medical Research Council (Project 03X-5915, 14X-09071,03X-217), the Swedish Association Against Chest and Heart Diseases, the foundation in Memory of Bengt Lundqvist, the Swedish Society for Medical Research, the Swedish Association Against Asthma and Allergy (RmA), the Swedish National Board for Laboratory Animals (CFN), the Scientific Council of the Swedish Association Against Use of Experimental Animals in Research, the Institute of Environmental Medicine, the Swedish Environment Protection Board (5324069-3), Karolinska Institutet and NM GM3 1278.
PY - 1990/7/16
Y1 - 1990/7/16
N2 - Exogenous [3H]leukotriene B4 (LTB4) was converted into several polar and non-polar metabolites in the chopped human lung. One of the major metabolites was identified as 5(S),12-dihydroxy-6,8,14-eicosatrienoic acid (10,11-dihydro-LTB4) by means of co-chromatography with authentic standards, ultraviolet spectrometry and gas chromatography-mass spectrometry. Analysis on chiral straight phase HPLC revealed the presence of both the 12(S) and 12(R) epimers of dihydro-LTB4. Dihydro-LTB4 was also formed from endogenously generated LTB4 in ionophore A23187 stimulated incubations. The dihydro metabolites were approximately 100 times less potent than LTB4 in causing guinea pig lung strip contraction and leukocyte-dependent inflammation in the hamster cheek pouch in vivo.
AB - Exogenous [3H]leukotriene B4 (LTB4) was converted into several polar and non-polar metabolites in the chopped human lung. One of the major metabolites was identified as 5(S),12-dihydroxy-6,8,14-eicosatrienoic acid (10,11-dihydro-LTB4) by means of co-chromatography with authentic standards, ultraviolet spectrometry and gas chromatography-mass spectrometry. Analysis on chiral straight phase HPLC revealed the presence of both the 12(S) and 12(R) epimers of dihydro-LTB4. Dihydro-LTB4 was also formed from endogenously generated LTB4 in ionophore A23187 stimulated incubations. The dihydro metabolites were approximately 100 times less potent than LTB4 in causing guinea pig lung strip contraction and leukocyte-dependent inflammation in the hamster cheek pouch in vivo.
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U2 - 10.1016/0006-291X(90)91235-K
DO - 10.1016/0006-291X(90)91235-K
M3 - Article
C2 - 2164805
AN - SCOPUS:0025040484
SN - 0006-291X
VL - 170
SP - 23
EP - 29
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -