Hyperspectral and multispectral imaging in digital and computational pathology: A systematic review [Invited]

Samuel Ortega, Martin Halicek, Himar Fabelo, Gustavo M. Callico, Baowei Fei

Research output: Contribution to journalReview articlepeer-review

55 Scopus citations


Hyperspectral imaging (HSI) and multispectral imaging (MSI) technologies have the potential to transform the fields of digital and computational pathology. Traditional digitized histopathological slides are imaged with RGB imaging. Utilizing HSI/MSI, spectral information across wavelengths within and beyond the visual range can complement spatial information for the creation of computer-aided diagnostic tools for both stained and unstained histological specimens. In this systematic review, we summarize the methods and uses of HSI/MSI for staining and color correction, immunohistochemistry, autofluorescence, and histopathological diagnostic research. Studies include hematology, breast cancer, head and neck cancer, skin cancer, and diseases of central nervous, gastrointestinal, and genitourinary systems. The use of HSI/MSI suggest an improvement in the detection of diseases and clinical practice compared with traditional RGB analysis, and brings new opportunities in histological analysis of samples, such as digital staining or alleviating the inter-laboratory variability of digitized samples. Nevertheless, the number of studies in this field is currently limited, and more research is needed to confirm the advantages of this technology compared to conventional imagery.

Original languageEnglish (US)
Pages (from-to)3195-3233
Number of pages39
JournalBiomedical Optics Express
Issue number6
StatePublished - Jun 1 2020

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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