HPLC Analysis of 6-Mercaptopurine and Metabolites in Extracellular Body Fluids

J. L. Rudy, J. C. Argyle, N. Winick, P. Van Dreal

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


A convenient HPLC assay, which allows for the simultaneous measurement in extracellular fluids of 6-mercaptopurine and four of its metabolites, 6-thioguanine, 6-mercaptopurine riboside, 6-thioxanthine and 6-thiouric acid is described. Solid phase extraction allows for the clean isolation of analytes from plasma, urine or cerebrospinal fluid. The simultaneous determination of 6-mercaptopurine and some of its major metabolites in extracellular fluids may contribute to the monitoring of patient compliance, bioavailability, and individual variation in metabolism and absorption.

Original languageEnglish (US)
Pages (from-to)504-509
Number of pages6
JournalAnnals of Clinical Biochemistry
Issue number5
StatePublished - Sep 1988

ASJC Scopus subject areas

  • Clinical Biochemistry


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