TY - JOUR
T1 - How microbiological tests reflect bacterial pathogenesis and host adaptation
AU - Spiga, Luisella
AU - Jimenez, Angel G.
AU - Santos, Renato L.
AU - Winter, Sebastian E.
N1 - Publisher Copyright:
© 2021, Sociedade Brasileira de Microbiologia.
PY - 2021/12
Y1 - 2021/12
N2 - Historically, clinical microbiological laboratories have often relied on isolation of pure cultures and phenotypic testing to identify microorganisms. These clinical tests are often based on specific biochemical reactions, growth characteristics, colony morphology, and other physiological aspects. The features used for identification in clinical laboratories are highly conserved and specific for a given group of microbes. We speculate that these features might be the result of evolutionary selection and thus may reflect aspects of the life cycle of the organism and pathogenesis. Indeed, several of the metabolic pathways targeted by diagnostic tests in some cases may represent mechanisms for host colonization or pathogenesis. Examples include, but are not restricted to, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella enterica, Shigella spp., and enteroinvasive Escherichia coli (EIEC). Here, we provide an overview of how some common tests reflect molecular mechanisms of bacterial pathogenesis.
AB - Historically, clinical microbiological laboratories have often relied on isolation of pure cultures and phenotypic testing to identify microorganisms. These clinical tests are often based on specific biochemical reactions, growth characteristics, colony morphology, and other physiological aspects. The features used for identification in clinical laboratories are highly conserved and specific for a given group of microbes. We speculate that these features might be the result of evolutionary selection and thus may reflect aspects of the life cycle of the organism and pathogenesis. Indeed, several of the metabolic pathways targeted by diagnostic tests in some cases may represent mechanisms for host colonization or pathogenesis. Examples include, but are not restricted to, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella enterica, Shigella spp., and enteroinvasive Escherichia coli (EIEC). Here, we provide an overview of how some common tests reflect molecular mechanisms of bacterial pathogenesis.
KW - Bacteria
KW - Bacterial culture
KW - Diagnostic tests
KW - Metabolism
KW - Pathogenesis
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U2 - 10.1007/s42770-021-00571-7
DO - 10.1007/s42770-021-00571-7
M3 - Review article
C2 - 34251610
AN - SCOPUS:85110248824
SN - 1517-8382
VL - 52
SP - 1745
EP - 1753
JO - Brazilian Journal of Microbiology
JF - Brazilian Journal of Microbiology
IS - 4
ER -