TY - JOUR
T1 - Homologous recognition of a promoter domain common to the MSV LTR and the HSV tk gene
AU - Graves, Barbara J.
AU - Johnson, Peter F.
AU - McKnight, Steven L.
N1 - Funding Information:
The dyad symmetry within dsll of the tk promoter that binds CBP was first noted by Stephen Eisenberg. We also thank Steven Triezenberg for critical comments on the manuscript, Bob Kingsbury for excellent technical assistance, Kathryn Jones and Robert Tjian for continuous and open communication of unpublished results, and Shirley Whitaker for clerical assistance. B. J. G. was supported by postdoctoral feilow-ships from the National institutes of Health and the Carnegie Corporation. P F J. was supported by a Damon Runyon-Walter Wincheil Fellowship. This work was otherwise funded by a grant from the National Institutes of Health to S. L. M.
PY - 1986/2/28
Y1 - 1986/2/28
N2 - We have partially purified, from rat liver, a nuclear protein fraction with sequence-specific affinity to a promoter domain shared by the herpesvirus tk gene and the Moloney murine sarcoma virus LTR. For both promoters, the protein-binding domain occurs roughly 80 bp upstream of the mRNA cap site and harbors the pentanucleotide sequence 5′-CCAAT-3′. The MSV LTR pentanucleotide occurs on the coding strand in an orientation pointing toward the retroviral transcription unit. The HSV tk pentanucleotide occurs on the non-coding strand in an orientation pointing away from the gene. Assays in microinjected frog oocytes and transfected mouse L cells indicate that equivalent point mutations, introduced at each residue of the CAT pentanucleotide of each promoter, lead to similar changes in promoter activity. Furthermore, they similarly alter binding of the nuclear protein fraction. Surprisingly, a C to G transversion at the first residue of the CAT pentanucleotide, which severely impairs the activity of both promoters, appears to increase affinity of the CAT binding protein.
AB - We have partially purified, from rat liver, a nuclear protein fraction with sequence-specific affinity to a promoter domain shared by the herpesvirus tk gene and the Moloney murine sarcoma virus LTR. For both promoters, the protein-binding domain occurs roughly 80 bp upstream of the mRNA cap site and harbors the pentanucleotide sequence 5′-CCAAT-3′. The MSV LTR pentanucleotide occurs on the coding strand in an orientation pointing toward the retroviral transcription unit. The HSV tk pentanucleotide occurs on the non-coding strand in an orientation pointing away from the gene. Assays in microinjected frog oocytes and transfected mouse L cells indicate that equivalent point mutations, introduced at each residue of the CAT pentanucleotide of each promoter, lead to similar changes in promoter activity. Furthermore, they similarly alter binding of the nuclear protein fraction. Surprisingly, a C to G transversion at the first residue of the CAT pentanucleotide, which severely impairs the activity of both promoters, appears to increase affinity of the CAT binding protein.
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U2 - 10.1016/0092-8674(86)90266-7
DO - 10.1016/0092-8674(86)90266-7
M3 - Article
C2 - 3004739
AN - SCOPUS:0022508422
SN - 0092-8674
VL - 44
SP - 565
EP - 576
JO - Cell
JF - Cell
IS - 4
ER -