TY - JOUR
T1 - HLA loss variants of a B27+ lymphoblastoid cell line
T2 - Genetic and cellular characterization
AU - Nicklas, Janice A.
AU - Miyachi, Yoshiki
AU - Taurog, Joel D.
AU - Wee, Siew Lin
AU - Chen, Li Kuang
AU - Grumet, F. Carl
AU - Bach, Fritz H.
N1 - Funding Information:
ACKNOWLEDGMENTS Drs. J. A. Nicklas and F. H. Bach were supportedb y grantsR O1 AI 17687,R O1 AI 19007,a nd 2 PO1 AM 13083f rom the NationalI nstituteso f Health,# R6 1505-A-1 fromt heN ationaMl ultipleS clerosisS ocietya, nd# A-188f romt heM archo f DimesB irth DefectF oundationD. r. Y. Miyachii s on leaveo f absencfer omthe Departmenotf Der-matologyF, aculty of Medicine,K yoto UniversityK, yoto,Japan. Dr. J. D. Taurog is supportebdy Young InvestigatoArw ardA M 29799f romt heN ationaIln stituteosf Health and by a grantf romthe Kroc FoundationD. r. S.-L. Wee is a recipienot f a SpecialF ellow Awardf romt heL eukemiSao cietyo f AmericaD. r. L.-K. Cheni s supportebdy t heM inistry of NationalD efenseo f the Republico f China. Dr. F. C. Grumetis supportedby grant HL 29572.
PY - 1984/9
Y1 - 1984/9
N2 - Variants of a lymphoblastoid cell line, LCL 526 (SB3 MB1 DR1 B44 C5 A2/SB4 MT4 DR4 B27 C2 A24), which lost various HLA specificities were selected with monoclonal antibodies and complement using a method developed by Kavathas et al. (PNAS 77:4251, 1980). Using αB27 monoclonals, 8 B27 only loss mutants and 4 B27 haplotype multiple loss mutants were generated. The parental LCL 526 and two of the B27- mutants were used to select αB27 CTLs. The selection of six A2 loss, one A2-C5 loss, and 14 A2 haplotype multiple loss variants as well as secondary selection on haplotype loss variants to obtain A null, B null, DR null, and total A,B,C, null variants is also described. The usefulness of these mutants for the study of the relationship between B27 and disease and as two new haplotypes for immunologic, genetic, and molecular research is discussed. These mutants are available to other researchers.
AB - Variants of a lymphoblastoid cell line, LCL 526 (SB3 MB1 DR1 B44 C5 A2/SB4 MT4 DR4 B27 C2 A24), which lost various HLA specificities were selected with monoclonal antibodies and complement using a method developed by Kavathas et al. (PNAS 77:4251, 1980). Using αB27 monoclonals, 8 B27 only loss mutants and 4 B27 haplotype multiple loss mutants were generated. The parental LCL 526 and two of the B27- mutants were used to select αB27 CTLs. The selection of six A2 loss, one A2-C5 loss, and 14 A2 haplotype multiple loss variants as well as secondary selection on haplotype loss variants to obtain A null, B null, DR null, and total A,B,C, null variants is also described. The usefulness of these mutants for the study of the relationship between B27 and disease and as two new haplotypes for immunologic, genetic, and molecular research is discussed. These mutants are available to other researchers.
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U2 - 10.1016/0198-8859(84)90053-3
DO - 10.1016/0198-8859(84)90053-3
M3 - Article
C2 - 6090351
AN - SCOPUS:0021612210
SN - 0198-8859
VL - 11
SP - 19
EP - 30
JO - Human Immunology
JF - Human Immunology
IS - 1
ER -