TY - JOUR
T1 - Histocompatibility antigen-activated cytotoxic T lymphocytes
T2 - II. Estimates of the frequency and specificity of precursors*
AU - Fischer Lindahl, K.
AU - Wilson, D. B.
PY - 1977/3/1
Y1 - 1977/3/1
N2 - Using limiting dilutions of responding cells in mouse mixed leukocyte cultures, we obtained direct estimates of the minimum frequency of precursors of cytotoxic T lymphocytes (CTL.P) for a variety of antigens. Depending on the strain combination, there were as many as 4-15 CTL. P reactive to DBA/2 among 104 lymph node cells. Taking into account that only 5-10% of peripheral T lymphocytes have the potential to develop into cytotoxic T lymphocytes (CTLs) (6), this implies that at least 1-2% of all CTL. P are responsive to any given H-2 haplotype difference. Precursors of cytotoxic cells thus have the same high frequency of cells reactive to alloantigens of the major histocompatibility complex as found among proliferating cells in graft-vs.-host reactions and mixed lymphocyte interactions. The frequencies of CTL. P reactive to xenoantigens (rat) or trinitrophenyl-modified self were less than half the frequency of alloreactive CTL.P. A minority of the CTL. P specific for one H-2 haplotype were also reactive to a third party H-2 haplotype, presumably on the basis of recognition of shared determinants. By dilution of sensitized cells from single microcultures, it was shown that a single CTL. P undergoes a minimum of three to four cell divisions and generates at least 8-16 CTLs after antigenic activation.
AB - Using limiting dilutions of responding cells in mouse mixed leukocyte cultures, we obtained direct estimates of the minimum frequency of precursors of cytotoxic T lymphocytes (CTL.P) for a variety of antigens. Depending on the strain combination, there were as many as 4-15 CTL. P reactive to DBA/2 among 104 lymph node cells. Taking into account that only 5-10% of peripheral T lymphocytes have the potential to develop into cytotoxic T lymphocytes (CTLs) (6), this implies that at least 1-2% of all CTL. P are responsive to any given H-2 haplotype difference. Precursors of cytotoxic cells thus have the same high frequency of cells reactive to alloantigens of the major histocompatibility complex as found among proliferating cells in graft-vs.-host reactions and mixed lymphocyte interactions. The frequencies of CTL. P reactive to xenoantigens (rat) or trinitrophenyl-modified self were less than half the frequency of alloreactive CTL.P. A minority of the CTL. P specific for one H-2 haplotype were also reactive to a third party H-2 haplotype, presumably on the basis of recognition of shared determinants. By dilution of sensitized cells from single microcultures, it was shown that a single CTL. P undergoes a minimum of three to four cell divisions and generates at least 8-16 CTLs after antigenic activation.
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U2 - 10.1084/jem.145.3.508
DO - 10.1084/jem.145.3.508
M3 - Article
C2 - 233899
AN - SCOPUS:0017619985
SN - 0022-1007
VL - 145
SP - 508
EP - 522
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 3
ER -