@inbook{1ec7eed29b2e46b7acdde4226331849c,
title = "Highly efficient derivation of pluripotent stem cells from mouse preimplantation and postimplantation embryos in serum-free conditions",
abstract = "Pluripotency refers to the potential of cells to generate all cell types of the embryo proper. Pluripotency spans a spectrum of cellular states. At one polar extreme is na{\"i}ve pluripotency, which is identified based on the potential to form germline chimeras. At the other polar extreme is primed pluripotency, in which pluripotent cells are primed to differentiate. Mouse na{\"i}ve PS cells can be derived from preimplantation embryos. Primed epiblast stem (EpiS) cells are typically isolated from epiblasts of early postimplantation mouse embryos. In this chapter, we describe protocols for highly efficient derivation and propagation of murine na{\"i}ve and primed PS cell lines in serum-free conditions from preimplantation and postimplantation embryos. We describe generation of mouse na{\"i}ve PS cells using LIF and inhibitors of MEK and GSK3 kinases and of mouse primed PS cells using FGF2 and IWR1 compound which induces the stabilization of Axin proteins.",
keywords = "Embryonic stem cells, Epiblast stem cells, Pluripotency, Pluripotent stem cells, Postimplantation epiblast, Preimplantation epiblast, Region-selective epiblast stem cells",
author = "{De Los Angeles}, Alejandro and Daiji Okamura and Jun Wu",
year = "2019",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-9524-0_2",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "29--36",
booktitle = "Methods in Molecular Biology",
}