High-throughput quantitative bioluminescence imaging for assessing tumor burden

Angelina Contero, Edmond Richer, Ana Gondim, Ralph P. Mason

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations


Bioluminescence imaging (BLI) has emerged during the past 5 years as the preeminent method for rapid, cheap, facile screening of tumor growth and spread in mice. Both subcutaneous and orthotopic tumor models are readily observed with high sensitivity and reproducibility. User-friendly commercial instruments exist and, increasingly, luciferase-expressing tumor cells are available in academic institutions or commercially. There is an increasing literature on routine use of BLI for assessing chemotherapeutic efficacy, drug combinations, dosing, and timing. In addition, BLI may be applied to more sophisticated questions of molecular biology by including specific promoter sequences. This chapter will describe routine methods used to support multiple investigators in our small animal imaging resource.

Original languageEnglish (US)
Title of host publicationBioluminescence
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages9
ISBN (Print)9781603273206
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • IGOR Pro
  • Luciferase
  • bioluminescence
  • charged-coupled device cameras (CCD)
  • luciferin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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