Abstract
Following cesarean section 102 women were treated with cefamandole by either perioperative intravenous administration or intraperitoneal irrigation. High-pressure liquid chromatographic (HPLC) methods for the quantitation of the low serum levels of cefamandole following intraperitoneal lavage were developed. The antibiotic was assayed in the serum using a standard microbiological assay and two types of reverse phase column technology for HPLC. The two HPLC systems were almost identical in performance. Both HPLC methods were at least 10-fold more sensitive than the microbiological assay. The correlation between the three methods was 0.9739. The half-life of cefamandole was 37 min, which was not significantly different from the half-life of the drug in serum of non-pregnant women. The peak serum levels were 47.6 ± 36.8 μg/ml and 1.98 ± 1.5 μg/ml for the intravenous and intraperitoneal methods of administration, respectively.
Original language | English (US) |
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Pages (from-to) | 2747-2759 |
Number of pages | 13 |
Journal | Journal of Liquid Chromatography |
Volume | 6 |
Issue number | 14 |
DOIs | |
State | Published - Dec 1 1983 |
ASJC Scopus subject areas
- Molecular Medicine