High and low doses of ionizing radiation induce different secretome profiles in a human skin model

Qibin Zhang, Melissa Matzke, Athena A. Schepmoes, Ronald J. Moore, Bobbie Jo Webb-Robertson, Zeping Hu, Matthew E. Monroe, Wei Jun Qian, Richard D. Smith, William F. Morgan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

It is postulated that secreted soluble factors are important contributors of bystander effect and adaptive responses observed in low dose ionizing radiation. Using multidimensional liquid chromatography-mass spectrometry based proteomics, we quantified the changes of skin tissue secretome - the proteins secreted from a full thickness, reconstituted 3-dimensional skin tissue model 48 hr after exposure to 3, 10 and 200 cGy of X-rays. Overall, 135 proteins showed statistical significant difference between the sham (0 cGy) and any of the irradiated groups (3, 10 or 200 cGy) on the basis of Dunnett adjusted t-test; among these, 97 proteins showed a trend of downregulation and 9 proteins showed a trend of upregulation with increasing radiation dose. In addition, there were 21 and 8 proteins observed to have irregular trends with the 10 cGy irradiated group either having the highest or the lowest level among all three radiated doses. Moreover, two proteins, carboxypeptidase E and ubiquitin carboxyl-terminal hydrolase isozyme L1 were sensitive to ionizing radiation, but relatively independent of radiation dose. Conversely, proteasome activator complex subunit 2 protein appeared to be sensitive to the dose of radiation, as rapid upregulation of this protein was observed when radiation doses were increased from 3, to 10 or 200 cGy. These results suggest that different mechanisms of action exist at the secretome level for low and high doses of ionizing radiation.

Original languageEnglish (US)
Article numbere92332
JournalPloS one
Volume9
Issue number3
DOIs
StatePublished - Mar 18 2014

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Agricultural and Biological Sciences
  • General

Fingerprint

Dive into the research topics of 'High and low doses of ionizing radiation induce different secretome profiles in a human skin model'. Together they form a unique fingerprint.

Cite this