TY - JOUR
T1 - Hepatic gene expression profiles associated with fibrosis progression and hepatocarcinogenesis in hepatitis C patients
AU - Shao, Run Xuan
AU - Hoshida, Yujin
AU - Otsuka, Motoyuki
AU - Kato, Naoya
AU - Tateishi, Ryosuke
AU - Teratani, Takuma
AU - Shiina, Shuichiro
AU - Taniguchi, Hiroyoshi
AU - Moriyama, Masaru
AU - Kawabe, Takao
AU - Omata, Masao
PY - 2005/4/7
Y1 - 2005/4/7
N2 - Aim: To determine fibrosis progression and hepatocellular carcinoma (HCC), using simultaneous gene expression analysis. Methods: Total RNA samples were extracted from liver biopsies from 19 patients with hepatitis C virus (HCV) infection and 3 patients without HCV infection. Among the 19 HCV-infected patients, 7 and 12 patients had grade F1-2 and F3-4 fibrosis, respectively. Of the 12 patients with F3-4 fibrosis, 8 had HCC. Gene expression in the liver samples was determined using an oligonucleotide microarray. The following comparisons were performed: normal livers vs HCV-infected livers; F1-2 vs F3-4; and F3-4 with HCC vs F3-4 without HCC. Genes that were differentially expressed between these groups were identified based on signal-to-noise ratios. Results: In the HCV-infected livers, genes involved in immune responses were highly expressed. Expression levels of genes for plasma proteins and drug-metabolizing enzymes were decreased and those of genes involved in the cell cycle and oncogenesis were increased in the F3-4 cases as compared to the F1-2 cases. Among the F3-4 cases, genes involved in carbohydrate metabolism tended to be more highly expressed in patients with HCC than in patients without HCC. Conclusion: We identified genes that are associated with fibrosis progression and hepatocarcinogenesis. This information may be used to detect increased carcinogenic potential in the livers of patients with HCV infection.
AB - Aim: To determine fibrosis progression and hepatocellular carcinoma (HCC), using simultaneous gene expression analysis. Methods: Total RNA samples were extracted from liver biopsies from 19 patients with hepatitis C virus (HCV) infection and 3 patients without HCV infection. Among the 19 HCV-infected patients, 7 and 12 patients had grade F1-2 and F3-4 fibrosis, respectively. Of the 12 patients with F3-4 fibrosis, 8 had HCC. Gene expression in the liver samples was determined using an oligonucleotide microarray. The following comparisons were performed: normal livers vs HCV-infected livers; F1-2 vs F3-4; and F3-4 with HCC vs F3-4 without HCC. Genes that were differentially expressed between these groups were identified based on signal-to-noise ratios. Results: In the HCV-infected livers, genes involved in immune responses were highly expressed. Expression levels of genes for plasma proteins and drug-metabolizing enzymes were decreased and those of genes involved in the cell cycle and oncogenesis were increased in the F3-4 cases as compared to the F1-2 cases. Among the F3-4 cases, genes involved in carbohydrate metabolism tended to be more highly expressed in patients with HCC than in patients without HCC. Conclusion: We identified genes that are associated with fibrosis progression and hepatocarcinogenesis. This information may be used to detect increased carcinogenic potential in the livers of patients with HCV infection.
KW - Hepatitis C
KW - Hepatocellular carcinoma
KW - Oligonucleotide microarray
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U2 - 10.3748/wjg.v11.i13.1995
DO - 10.3748/wjg.v11.i13.1995
M3 - Article
C2 - 15800993
AN - SCOPUS:20244373050
SN - 1007-9327
VL - 11
SP - 1995
EP - 1999
JO - World Journal of Gastroenterology
JF - World Journal of Gastroenterology
IS - 13
ER -