Hair follicle stem cells provide a functional niche for melanocyte stem cells

Shintaro Tanimura; Yuko Tadokoro; Ken Inomata; Nguyen Thanh Binh; Wataru Nishie; Satoshi Yamazaki; Hiromitsu Nakauchi; Yoshio Tanaka; James R. McMillan; Daisuke Sawamura; Kim Yancey; Hiroshi Shimizu; Emi K. Nishimura

doi:10.1016/j.stem.2010.11.029

Hair follicle stem cells provide a functional niche for melanocyte stem cells

Shintaro Tanimura, Yuko Tadokoro, Ken Inomata, Nguyen Thanh Binh, Wataru Nishie, Satoshi Yamazaki, Hiromitsu Nakauchi, Yoshio Tanaka, James R. McMillan, Daisuke Sawamura, Kim Yancey, Hiroshi Shimizu, Emi K. Nishimura

Research output: Contribution to journal › Article › peer-review

219 Scopus citations

Abstract

In most stem cell systems, the organization of the stem cell niche and the anchoring matrix required for stem cell maintenance are largely unknown. We report here that collagen XVII (COL17A1/BP180/BPAG2), a hemidesmosomal transmembrane collagen, is highly expressed in hair follicle stem cells (HFSCs) and is required for the maintenance not only of HFSCs but also of melanocyte stem cells (MSCs), which do not express Col17a1 but directly adhere to HFSCs. Mice lacking Col17a1 show premature hair graying and hair loss. Analysis of Col17a1-null mice revealed that COL17A1 is critical for the self-renewal of HFSCs through maintaining their quiescence and immaturity, potentially explaining the mechanism underlying hair loss in human COL17A1 deficiency. Moreover, forced expression of COL17A1 in basal keratinocytes, including HFSCs, in Col17a1-null mice rescues MSCs from premature differentiation and restores TGF-β signaling, demonstrating that HFSCs function as a critical regulatory component of the MSC niche.

Original language	English (US)
Pages (from-to)	177-187
Number of pages	11
Journal	Cell Stem Cell
Volume	8
Issue number	2
DOIs	https://doi.org/10.1016/j.stem.2010.11.029
State	Published - Feb 4 2011

ASJC Scopus subject areas

Molecular Medicine
Genetics
Cell Biology

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10.1016/j.stem.2010.11.029

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@article{a95b2ccf4df846e1882795fd25be375f,

title = "Hair follicle stem cells provide a functional niche for melanocyte stem cells",

abstract = "In most stem cell systems, the organization of the stem cell niche and the anchoring matrix required for stem cell maintenance are largely unknown. We report here that collagen XVII (COL17A1/BP180/BPAG2), a hemidesmosomal transmembrane collagen, is highly expressed in hair follicle stem cells (HFSCs) and is required for the maintenance not only of HFSCs but also of melanocyte stem cells (MSCs), which do not express Col17a1 but directly adhere to HFSCs. Mice lacking Col17a1 show premature hair graying and hair loss. Analysis of Col17a1-null mice revealed that COL17A1 is critical for the self-renewal of HFSCs through maintaining their quiescence and immaturity, potentially explaining the mechanism underlying hair loss in human COL17A1 deficiency. Moreover, forced expression of COL17A1 in basal keratinocytes, including HFSCs, in Col17a1-null mice rescues MSCs from premature differentiation and restores TGF-β signaling, demonstrating that HFSCs function as a critical regulatory component of the MSC niche.",

author = "Shintaro Tanimura and Yuko Tadokoro and Ken Inomata and Binh, {Nguyen Thanh} and Wataru Nishie and Satoshi Yamazaki and Hiromitsu Nakauchi and Yoshio Tanaka and McMillan, {James R.} and Daisuke Sawamura and Kim Yancey and Hiroshi Shimizu and Nishimura, {Emi K.}",

note = "Funding Information: We thank Dr. Makoto Taketo for Tgfbr2 knockout mice; Dr. Hideki Nakamura, Dr. Tomohiko Wakayama, and Dr. Shoichi Iseki for their technical advice concerning electron microscopic analysis; Dr. Hiroyuki Nishimura for critical reading of the manuscript; Dr. Masashi Akiyama for discussion; Dr. Atsushi Hirao and Dr. Masako Ohmura for the use of the flow cytometer; Dr. Ken Natsuga and Ms. Kaori Sakai for sample identification; and Ms. Misa Suzuki, Ms. Megumi Sato, and Ms. Yuika Osaki for technical assistance. This study was supported by grants from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (17689033, 19390293), the Uehara Memorial Foundation, the Kato Memorial Bioscience Foundation, and the Takeda Science Foundation to E.K.N. ",

year = "2011",

month = feb,

day = "4",

doi = "10.1016/j.stem.2010.11.029",

language = "English (US)",

volume = "8",

pages = "177--187",

journal = "Cell Stem Cell",

issn = "1934-5909",

publisher = "Cell Press",

number = "2",

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T1 - Hair follicle stem cells provide a functional niche for melanocyte stem cells

AU - Tanimura, Shintaro

AU - Tadokoro, Yuko

AU - Inomata, Ken

AU - Binh, Nguyen Thanh

AU - Nishie, Wataru

AU - Yamazaki, Satoshi

AU - Nakauchi, Hiromitsu

AU - Tanaka, Yoshio

AU - McMillan, James R.

AU - Sawamura, Daisuke

AU - Yancey, Kim

AU - Shimizu, Hiroshi

AU - Nishimura, Emi K.

N1 - Funding Information: We thank Dr. Makoto Taketo for Tgfbr2 knockout mice; Dr. Hideki Nakamura, Dr. Tomohiko Wakayama, and Dr. Shoichi Iseki for their technical advice concerning electron microscopic analysis; Dr. Hiroyuki Nishimura for critical reading of the manuscript; Dr. Masashi Akiyama for discussion; Dr. Atsushi Hirao and Dr. Masako Ohmura for the use of the flow cytometer; Dr. Ken Natsuga and Ms. Kaori Sakai for sample identification; and Ms. Misa Suzuki, Ms. Megumi Sato, and Ms. Yuika Osaki for technical assistance. This study was supported by grants from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (17689033, 19390293), the Uehara Memorial Foundation, the Kato Memorial Bioscience Foundation, and the Takeda Science Foundation to E.K.N.

PY - 2011/2/4

Y1 - 2011/2/4

N2 - In most stem cell systems, the organization of the stem cell niche and the anchoring matrix required for stem cell maintenance are largely unknown. We report here that collagen XVII (COL17A1/BP180/BPAG2), a hemidesmosomal transmembrane collagen, is highly expressed in hair follicle stem cells (HFSCs) and is required for the maintenance not only of HFSCs but also of melanocyte stem cells (MSCs), which do not express Col17a1 but directly adhere to HFSCs. Mice lacking Col17a1 show premature hair graying and hair loss. Analysis of Col17a1-null mice revealed that COL17A1 is critical for the self-renewal of HFSCs through maintaining their quiescence and immaturity, potentially explaining the mechanism underlying hair loss in human COL17A1 deficiency. Moreover, forced expression of COL17A1 in basal keratinocytes, including HFSCs, in Col17a1-null mice rescues MSCs from premature differentiation and restores TGF-β signaling, demonstrating that HFSCs function as a critical regulatory component of the MSC niche.

AB - In most stem cell systems, the organization of the stem cell niche and the anchoring matrix required for stem cell maintenance are largely unknown. We report here that collagen XVII (COL17A1/BP180/BPAG2), a hemidesmosomal transmembrane collagen, is highly expressed in hair follicle stem cells (HFSCs) and is required for the maintenance not only of HFSCs but also of melanocyte stem cells (MSCs), which do not express Col17a1 but directly adhere to HFSCs. Mice lacking Col17a1 show premature hair graying and hair loss. Analysis of Col17a1-null mice revealed that COL17A1 is critical for the self-renewal of HFSCs through maintaining their quiescence and immaturity, potentially explaining the mechanism underlying hair loss in human COL17A1 deficiency. Moreover, forced expression of COL17A1 in basal keratinocytes, including HFSCs, in Col17a1-null mice rescues MSCs from premature differentiation and restores TGF-β signaling, demonstrating that HFSCs function as a critical regulatory component of the MSC niche.

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U2 - 10.1016/j.stem.2010.11.029

DO - 10.1016/j.stem.2010.11.029

M3 - Article

C2 - 21295274

AN - SCOPUS:79551588964

SN - 1934-5909

VL - 8

SP - 177

EP - 187

JO - Cell Stem Cell

JF - Cell Stem Cell

IS - 2

ER -

Hair follicle stem cells provide a functional niche for melanocyte stem cells

Abstract

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