Glutamate Release Machinery Is Altered in the Frontal Cortex of Rats with Experimental Autoimmune Encephalomyelitis

Natalí L. Chanaday, A. Alejandro Vilcaes, Ana L. de Paul, Alicia I. Torres, Alicia L. Degano, German A. Roth

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Experimental autoimmune encephalomyelitis (EAE) is an animal model that mimics many of the clinical and pathological features of the human disease multiple sclerosis (MS). Both are inflammatory demyelinating and neurodegenerative pathologies of the central nervous system associated with motor, sensory, and cognitive deficits. In MS, gray matter atrophy is related to the emergence of cognitive deficits and contributes to clinical progression. In particular, prefrontal cortex injury and dysfunction have been correlated to the development of fatigue, one of the most common and disabling symptoms in MS. However, the molecular bases of these changes remain unknown. Taking advantage of EAE similitude, we herein analyze functional and morphological changes in isolated cortical presynaptic terminals (synaptosomes) from an acute rat model. We found impaired glutamate release in the frontal cortex from EAE rats. This defect appeared along with the onset of the disease, reversing when clinical signs were no more evident. Biochemical analysis of EAE synaptosomes revealed alterations in the presynaptic release machinery and in the response to depolarization, which was accompanied by abnormal synapsin I phosphorylation and dispersion. These changes were associated with reduced synaptic vesicle mobility, with no alterations in synaptosomal morphology as evidenced by electron microscopy. The present are the first pieces of evidence unraveling the molecular mechanisms of frontal cortex neuronal dysfunction in EAE and, possibly, MS.

Original languageEnglish (US)
Pages (from-to)1353-1367
Number of pages15
JournalMolecular Neurobiology
Issue number3
StatePublished - Jun 1 2015
Externally publishedYes


  • Experimental autoimmune encephalomyelitis
  • Glutamate release
  • Multiple sclerosis
  • Release machinery
  • Synapsin
  • Synaptosomes

ASJC Scopus subject areas

  • Neurology
  • Cellular and Molecular Neuroscience


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