TY - JOUR
T1 - Global shapes of F-actin depolymerization-competent minimal gelsolins
T2 - Insight into the role of g2-g3 linker in pH/Ca2+ insensitivity of the first half
AU - Peddada, Nagesh
AU - Sagar, Amin
AU - Rathore, Yogendra S.
AU - Choudhary, Vikas
AU - Pattnaik, U. Bharat K.
AU - Khatri, Neeraj
AU - Garg, Renu
AU - Ashish,
PY - 2013/9/27
Y1 - 2013/9/27
N2 - Background: Shape-function studies are necessary to design better therapeutic alternatives of the plasma gelsolin. Results: N-terminal fragment 30-161 is the smallest segment with F-actin depolymerization potential, and G1-G3 can function independent of Ca2+ ions or low pH. Conclusion: The g2-g3 linker plays a role in imparting pH/Ca2+ insensitivity to G1-G3. Significance: We provide the first evidence that g2-g3 linker regulates mobility of the G1 domain.
AB - Background: Shape-function studies are necessary to design better therapeutic alternatives of the plasma gelsolin. Results: N-terminal fragment 30-161 is the smallest segment with F-actin depolymerization potential, and G1-G3 can function independent of Ca2+ ions or low pH. Conclusion: The g2-g3 linker plays a role in imparting pH/Ca2+ insensitivity to G1-G3. Significance: We provide the first evidence that g2-g3 linker regulates mobility of the G1 domain.
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U2 - 10.1074/jbc.M113.463224
DO - 10.1074/jbc.M113.463224
M3 - Article
C2 - 23940055
AN - SCOPUS:84884777792
SN - 0021-9258
VL - 288
SP - 28266
EP - 28282
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 39
ER -