@article{7f06aac4256c4786a8a003887d8f11e1,
title = "Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection",
abstract = "To identify potential therapeutic targets for SARS-CoV-2 and related pathogenic coronaviruses, Wei et al. conduct genome-wide CRISPR screens in Vero-E6 cells using SARS-CoV-2, MERS-CoV, and pseudoviruses presenting SARS-CoV-1 or SARS-CoV-2 spike proteins. They identify pro-viral genes and pathways, including HMGB1 and the SWI/SNF chromatin remodeling complex, that are SARS lineage and pan-coronavirus specific, respectively, and demonstrate that HMGB1 is critical for SARS lineage viral entry because it has a critical role in ACE2 expression.",
keywords = "COVID-19, CRISPR screen, Epigenetics, HMGB1, MERS-CoV, Middle East Respiratory Syndrome, SARS-CoV-2, SWI/SNF complex, Severe Acute Respiratory Syndrome",
author = "Jin Wei and Alfajaro, {Mia Madel} and DeWeirdt, {Peter C.} and Hanna, {Ruth E.} and Lu-Culligan, {William J.} and Cai, {Wesley L.} and Strine, {Madison S.} and Zhang, {Shang Min} and Graziano, {Vincent R.} and Schmitz, {Cameron O.} and Chen, {Jennifer S.} and Mankowski, {Madeleine C.} and Filler, {Renata B.} and Ravindra, {Neal G.} and Victor Gasque and {de Miguel}, {Fernando J.} and Ajinkya Patil and Huacui Chen and Oguntuyo, {Kasopefoluwa Y.} and Laura Abriola and Surovtseva, {Yulia V.} and Orchard, {Robert C.} and Benhur Lee and Lindenbach, {Brett D.} and Katerina Politi and {van Dijk}, David and Cigall Kadoch and Simon, {Matthew D.} and Qin Yan and Doench, {John G.} and Wilen, {Craig B.}",
note = "Funding Information: We would like to acknowledge Brian Smith, Nancy Brown, and Ruth Montgomery for generous resources; Douglas Brackney, Sean Whelan, Hyeryun Choe, Vincent Munster, Bart Haagmans, Paulina Pawlica, Joan Steitz, Jacco Boon, Pei-Yong Shi, Kenneth Plante, the World Reference Center for Emerging Viruses and Arboviruses (WRECVA), and BEI Resources for critical reagents; Marissa Feeley, Yenarae Lee, and the Yale Center for Genome Analysis (YCGA) for technical assistance and sample processing; Stephanie Eisenbarth, David Schatz, Richard Flavell, Scott Pope, and Ruslan Medzhitov for helpful discussions; and Benjamin Fontes and Daniele Scavone for Environmental Health and Safety. This work was supported by NIH grants K08 AI128043 (to C.B.W.), R01 AI148467 (to C.B.W.), U19 AI133524 (to J.G.D.), R21 AI157835 (to J.G.D.), P50 CA121974 (to Q.Y.), P50 CA196530 (to K.P.), R01 AI087925 (to B.D.L.), T32GM007223 (to W.J.L.-C.), T32GM007205 (to W.J.L.-C. and J.S.C.), F30HL149151 (to J.S.C.), R01 AI123449 (to B.L.), and F31 AI54739 (to K.O.); NSF fellowships DGE1122492 (to W.J.L.-C.) and DGE1752134 (to M.S.S.); a Burroughs Wellcome Fund Career Award for Medical Scientists (to C.B.W.); the Ludwig Family Foundation (to C.B.W.), the Mathers Charitable Foundation (to C.B.W.); an Emergent Ventures fast grant (to C.B.W. and D.v.D.); and a Merkin Institute fellowship (to J.G.D.). Funding Information: We would like to acknowledge Brian Smith, Nancy Brown, and Ruth Montgomery for generous resources; Douglas Brackney, Sean Whelan, Hyeryun Choe, Vincent Munster, Bart Haagmans, Paulina Pawlica, Joan Steitz, Jacco Boon, Pei-Yong Shi, Kenneth Plante, the World Reference Center for Emerging Viruses and Arboviruses (WRECVA), and BEI Resources for critical reagents; Marissa Feeley, Yenarae Lee, and the Yale Center for Genome Analysis (YCGA) for technical assistance and sample processing; Stephanie Eisenbarth, David Schatz, Richard Flavell, Scott Pope, and Ruslan Medzhitov for helpful discussions; and Benjamin Fontes and Daniele Scavone for Environmental Health and Safety. This work was supported by NIH grants K08 AI128043 (to C.B.W.), R01 AI148467 (to C.B.W.), U19 AI133524 (to J.G.D.), R21 AI157835 (to J.G.D.), P50 CA121974 (to Q.Y.), P50 CA196530 (to K.P.), R01 AI087925 (to B.D.L.), T32GM007223 (to W.J.L.-C.), T32GM007205 (to W.J.L.-C. and J.S.C.), F30HL149151(to J.S.C.), R01 AI123449 (to B.L.), and F31 AI54739 (to K.O.); NSF fellowships DGE1122492 (to W.J.L.-C.) and DGE1752134 (to M.S.S.); a Burroughs Wellcome Fund Career Award for Medical Scientists (to C.B.W.); the Ludwig Family Foundation (to C.B.W.), the Mathers Charitable Foundation (to C.B.W.); an Emergent Ventures fast grant (to C.B.W. and D.v.D.); and a Merkin Institute fellowship (to J.G.D.). Conceptualization, J.W. M.M.A. R.C.O. M.D.S. Q.Y. J.G.D. and C.B.W.; Methodology, J.W. M.M.A. R.E.H. W.J.L.-C. W.L.C. S.-M.Z. N.G.R. V.G. R.C.O. B.L. Q.Y. M.D.S. J.G.D. and C.B.W.; Investigation, J.W. M.M.A. W.J.L.-C. W.L.C. M.S.S. S.-M.Z. L.A. Y.V.S. C.O.S. J.S.C. V.R.G. M.C.M. R.B.F. and C.B.W.; Validation, J.W. M.M.A. W.J.L.-C. W.L.C. M.S.S. S.-M.Z. C.O.S. J.S.C. V.R.G. M.C.M. F.J.d.M. and H.C.; Formal Analysis, J.W. M.M.A. R.E.H. P.C.D. W.J.L.-C. W.L.C. M.S.S. N.G.R. V.G. A.P. D.v.D. C.K. J.G.D. and C.B.W.; Visualization, J.W. R.E.H. and P.C.D.; Resources, K.Y.O. B.L. K.P. D.v.D. C.K. J.G.D. and C.B.W.; Data Curation, R.E.H. and P.C.D.; Writing, J.W. P.C.D. R.E.H. J.G.D. and C.B.W.; Supervision, B.L. K.P. D.v.D. C.K. M.D.S. Q.Y. J.G.D. and C.B.W.; Funding Acquisition, J.G.D. C.B.W. Yale University (C.B.W.) has a patent pending related to this work entitled ?Compounds and Compositions for Treating, Ameliorating, and/or Preventing SARS-CoV-2 Infection and/or Complications Thereof.? Yale University has committed to rapidly executable non-exclusive royalty-free licenses to intellectual property rights for the purpose of making and distributing products to prevent, diagnose, and treat COVID-19 infection during the pandemic and for a short period thereafter. J.G.D. consults for Foghorn Therapeutics, Maze Therapeutics, Merck, Agios, and Pfizer. J.G.D. consults for and has equity in Tango Therapeutics. C.K. is the Scientific Founder, Board of Directors member, Scientific Advisory Board member, shareholder, and consultant for Foghorn Therapeutics, Inc. (Cambridge, MA). Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2021",
month = jan,
day = "7",
doi = "10.1016/j.cell.2020.10.028",
language = "English (US)",
volume = "184",
pages = "76--91.e13",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",
}