@article{7f06aac4256c4786a8a003887d8f11e1,
title = "Genome-wide CRISPR Screens Reveal Host Factors Critical for SARS-CoV-2 Infection",
abstract = "To identify potential therapeutic targets for SARS-CoV-2 and related pathogenic coronaviruses, Wei et al. conduct genome-wide CRISPR screens in Vero-E6 cells using SARS-CoV-2, MERS-CoV, and pseudoviruses presenting SARS-CoV-1 or SARS-CoV-2 spike proteins. They identify pro-viral genes and pathways, including HMGB1 and the SWI/SNF chromatin remodeling complex, that are SARS lineage and pan-coronavirus specific, respectively, and demonstrate that HMGB1 is critical for SARS lineage viral entry because it has a critical role in ACE2 expression.",
keywords = "COVID-19, CRISPR screen, Epigenetics, HMGB1, MERS-CoV, Middle East Respiratory Syndrome, SARS-CoV-2, SWI/SNF complex, Severe Acute Respiratory Syndrome",
author = "Jin Wei and Alfajaro, {Mia Madel} and DeWeirdt, {Peter C.} and Hanna, {Ruth E.} and Lu-Culligan, {William J.} and Cai, {Wesley L.} and Strine, {Madison S.} and Zhang, {Shang Min} and Graziano, {Vincent R.} and Schmitz, {Cameron O.} and Chen, {Jennifer S.} and Mankowski, {Madeleine C.} and Filler, {Renata B.} and Ravindra, {Neal G.} and Victor Gasque and {de Miguel}, {Fernando J.} and Ajinkya Patil and Huacui Chen and Oguntuyo, {Kasopefoluwa Y.} and Laura Abriola and Surovtseva, {Yulia V.} and Orchard, {Robert C.} and Benhur Lee and Lindenbach, {Brett D.} and Katerina Politi and {van Dijk}, David and Cigall Kadoch and Simon, {Matthew D.} and Qin Yan and Doench, {John G.} and Wilen, {Craig B.}",
note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2021",
month = jan,
day = "7",
doi = "10.1016/j.cell.2020.10.028",
language = "English (US)",
volume = "184",
pages = "76--91.e13",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1",
}