Genome-wide analysis and functional prediction of the estrogen-regulated transcriptional response in the mouse uterus

Yasmin M. Vasquez, Tulip S. Nandu, Andrew M. Kelleher, Enrique I. Ramos, Shrikanth S. Gadad, W. Lee Kraus

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The ovarian hormones estrogen and progesterone orchestrate the transcriptional programs required to direct functions of the uterus for initiation and maintenance of pregnancy. Estrogen, acting via estrogen receptor alpha, regulates gene expression by activating and repressing distinct genes involved in signaling pathways that regulate cellular and physiological responses including cell division, water influx, and immune cell recruitment. Historically, these transcriptional responses have been postulated to reflect a biphasic physiological response. In this study, we explored the transcriptional responses of the ovariectomized mouse uterus to 17β-estradiol (E2) by RNA-seq to obtain global expression profiles of protein-coding transcripts (mRNAs) and long noncoding RNAs (lncRNAs) following 0.5, 1, 2, and 6 hours of treatment. The E2-regulated mRNA and lncRNA expression profiles in the mouse uterus indicate an association between lncRNAs and mRNAs that regulate E2-driven pathways and reproductive phenotypes in the mouse. The transient E2-regulated transcriptome is reflected in the time-dependent shifting of biological processes regulated in the uterus in response to E2. Moreover, high expression of some conserved lncRNAs that are E2 regulated in the mouse uterus are predictive of low overall survival in endometrial carcinoma patients (e.g., H19, KCNQ1OT1, MIR17HG, and FTX). Collectively, this study (1) describes a genomic approach for identifying E2-regulated lncRNAs that may serve critical function in the uterus and (2) provides new insights into our understanding of the regulation of hormone-regulated transcriptional responses with implications in pregnancy and endometrial pathologies.

Original languageEnglish (US)
Pages (from-to)327-338
Number of pages12
JournalBiology of reproduction
Issue number2
StatePublished - Feb 14 2020


  • estradiol/estradiol receptor
  • gene expression
  • genomics
  • transcriptional regulation
  • uterus

ASJC Scopus subject areas

  • Reproductive Medicine
  • Cell Biology


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