Generation of superoxide anion by brain endothelial cell xanthine oxidase

Lance S. Terada, Irene R. Willingham, Mary E. Rosandich, Jonathan A. Leff, Glenn W. Kindt, John E. Repine

Research output: Contribution to journalArticlepeer-review

85 Scopus citations


Bovine brain endothelial cells (EC) that were isolated and propagated in pure culture had increased ( 20‐fold) levels of xanthine oxidase and xanthine dehydrogenase activity compared to whole brain homogenate. Brain EC also released superoxide anion (O 2−) in to the extracellular medium. Treatment of EC with tungsten decreased (P < 0.05), both XO activity and O 2− release. XO appears to be highly concentrated in cerebral vascular endothelium and may be an important source o; O 2−.

Original languageEnglish (US)
Pages (from-to)191-196
Number of pages6
JournalJournal of cellular physiology
Issue number2
StatePublished - Aug 1991

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology


Dive into the research topics of 'Generation of superoxide anion by brain endothelial cell xanthine oxidase'. Together they form a unique fingerprint.

Cite this