TY - JOUR
T1 - Gene organization and recombinational hotspots in the murine major histocompatibility complex
AU - Steinmetz, Michael
AU - Stephan, Danielle
AU - Lindahl, Kirsten Fischer
N1 - Funding Information:
We thank A. Rich for bringing to our attention the relationship of the Ea repeat sequence to DNA sequences potentially forming Z-DNA, M. Essrich for assistance in some of the Southern and Northern blot experiments, D. Larhammar for the communication of unpublished results, C. Steinberg and J. Kaufman for critical reading of the manuscript, H. P Stahlberger for artwork, and L. Gisler and J. Hossmann for the preparation of the manuscript. The Base1 institute for Immunology was founded and is supported by F. Hoffmann-La Roche, Inc., Basel, Switzerland.
PY - 1986/3/28
Y1 - 1986/3/28
N2 - By chromosome walking in the major histocompatibility complex (MHC) of the BALB c mouse, we have linked the K region to the I region at the molecular level. Forty-nine overlapping cosmid clones define a stretch of about 600 kb of DNA containing 2 class I and 7 class II genes. Eleven independent recombination events were mapped between the K and the I region marker loci by Southern blot analysis of polymorphic restriction sites. Eight of these events involved crossing-over, at an unusually high frequency of 0.6%-1.5%, between genes from Mus musculus castaneus and laboratory mouse strains, and they were confined to two small stretches of DNA. We conclude that recombination hotspots are present at these positions in the two M.m. castaneus MHC haplotypes tested. In contrast, several MHC haplotypes of laboratory mice appear to lack those hotspots.
AB - By chromosome walking in the major histocompatibility complex (MHC) of the BALB c mouse, we have linked the K region to the I region at the molecular level. Forty-nine overlapping cosmid clones define a stretch of about 600 kb of DNA containing 2 class I and 7 class II genes. Eleven independent recombination events were mapped between the K and the I region marker loci by Southern blot analysis of polymorphic restriction sites. Eight of these events involved crossing-over, at an unusually high frequency of 0.6%-1.5%, between genes from Mus musculus castaneus and laboratory mouse strains, and they were confined to two small stretches of DNA. We conclude that recombination hotspots are present at these positions in the two M.m. castaneus MHC haplotypes tested. In contrast, several MHC haplotypes of laboratory mice appear to lack those hotspots.
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U2 - 10.1016/0092-8674(86)90012-7
DO - 10.1016/0092-8674(86)90012-7
M3 - Article
C2 - 3754180
AN - SCOPUS:0022486989
SN - 0092-8674
VL - 44
SP - 895
EP - 904
JO - Cell
JF - Cell
IS - 6
ER -