TY - JOUR
T1 - Gene fusion analysis of membrane protein topology
T2 - A direct comparison of alkaline phosphatase and β-lactamase fusions
AU - Prinz, W. A.
AU - Beckwith, J.
PY - 1994
Y1 - 1994
N2 - To compare two approaches to analyzing membrane protein topology, a number of alkaline phosphatase fusions to membrane proteins were converted to β- lactamase fusions. While some alkaline phosphatase fusions near the N terminus of cytoplasmic loops of membrane proteins have anomalously high levels of activity, the equivalent β-lactamase fusions do not. This disparity may reflect differences in the folding of β-lactamase and alkaline phosphatase in the cytoplasm.
AB - To compare two approaches to analyzing membrane protein topology, a number of alkaline phosphatase fusions to membrane proteins were converted to β- lactamase fusions. While some alkaline phosphatase fusions near the N terminus of cytoplasmic loops of membrane proteins have anomalously high levels of activity, the equivalent β-lactamase fusions do not. This disparity may reflect differences in the folding of β-lactamase and alkaline phosphatase in the cytoplasm.
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U2 - 10.1128/jb.176.20.6410-6413.1994
DO - 10.1128/jb.176.20.6410-6413.1994
M3 - Comment/debate
C2 - 7929016
AN - SCOPUS:0028148705
SN - 0021-9193
VL - 176
SP - 6410
EP - 6413
JO - Journal of bacteriology
JF - Journal of bacteriology
IS - 20
ER -