G protein complement of SV40-transformed ciliary epithelial cells

Harold S. Cooper; David R. Manning; Martin B. Wax

doi:10.3109/02713689008999615

G protein complement of SV40-transformed ciliary epithelial cells

Harold S. Cooper, David R. Manning, Martin B. Wax

Ophthalmology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes G_sα and all three members of the G_iα family. Neither cell type contains G_oα or G_zα. Both cell lines contain β₃₅ and β₃₆. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.

Original language	English (US)
Pages (from-to)	493-499
Number of pages	7
Journal	Current Eye Research
Volume	9
Issue number	5
DOIs	https://doi.org/10.3109/02713689008999615
State	Published - 1990

ASJC Scopus subject areas

Ophthalmology
Sensory Systems
Cellular and Molecular Neuroscience

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title = "G protein complement of SV40-transformed ciliary epithelial cells",

abstract = "Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes Gsα and all three members of the Giα family. Neither cell type contains Goα or Gzα. Both cell lines contain β35 and β36. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.",

author = "Cooper, {Harold S.} and Manning, {David R.} and Wax, {Martin B.}",

note = "Funding Information: ACKNOWLEDGEMENTS This research was supported by grants EY 06810 (M.B.W.) and CA 39712 (D.R.M.) . H.S. Cooper i s a r e c i p i e n t of a N . I . H . Medical Scientists Training Program Fellowship and a Pharmaceutical Manufacturer's Association Foundation Pre-Doctoral Fellowship.",

year = "1990",

doi = "10.3109/02713689008999615",

language = "English (US)",

volume = "9",

pages = "493--499",

journal = "Current Eye Research",

issn = "0271-3683",

publisher = "Informa Healthcare",

number = "5",

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TY - JOUR

T1 - G protein complement of SV40-transformed ciliary epithelial cells

AU - Cooper, Harold S.

AU - Manning, David R.

AU - Wax, Martin B.

N1 - Funding Information: ACKNOWLEDGEMENTS This research was supported by grants EY 06810 (M.B.W.) and CA 39712 (D.R.M.) . H.S. Cooper i s a r e c i p i e n t of a N . I . H . Medical Scientists Training Program Fellowship and a Pharmaceutical Manufacturer's Association Foundation Pre-Doctoral Fellowship.

PY - 1990

Y1 - 1990

N2 - Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes Gsα and all three members of the Giα family. Neither cell type contains Goα or Gzα. Both cell lines contain β35 and β36. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.

AB - Guanine nucleotide-binding proteins (G proteins) are a family of receptor-coupled signal-transducing proteins that regulate a variety of second-messenger systems and ion channels. The complement of G proteins in SV40-transformed pigmented and nonpigmented ciliary epithelial cells was determined by Western blot analysis utilizing peptide and holoprotein derived antisera to known G protein α and β subunits and cholera toxin catalyzed ADP-ribosylation. The complement of α subunits found in both SV40-transformed NPE and PE cells includes Gsα and all three members of the Giα family. Neither cell type contains Goα or Gzα. Both cell lines contain β35 and β36. Future studies will examine the functional involvement of these G proteins in the regulation of aqueous humor stimulus-secretion coupling.

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G protein complement of SV40-transformed ciliary epithelial cells

Abstract

ASJC Scopus subject areas

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