TY - JOUR
T1 - FV peptide induces apoptosis in HEp 2 and HeLa cells
T2 - An insight into the mechanism of induction
AU - Sri Balasubashini, M.
AU - Karthigayan, S.
AU - Somasundaram, S. T.
AU - Balasubramanian, T.
AU - Rukkumani, R.
AU - Menon, Venugopal P.
PY - 2006/12/1
Y1 - 2006/12/1
N2 - The present study is an attempt to evaluate the antiproliferative potential of peptide (7.6 kDa) from lionfish (Pterios volitans) venom on cultured HEp2 and HeLa cells. Different dose of purified peptide (1, 2 and 4 μg/ml) at different time points (12, 24 and 36 hrs) were tested for antiproliferative index of the peptide. Among them, 2 μg/ml at 24 hrs was found to effectively inhibit cancer cell growth in vitro and did not cause any adverse effect on normal human lymphocytes. Apoptosis was examined by propidium iodide staining, confirmed by the expression of caspase-8 and caspase-3, down regulation of Bcl-2 expression and DNA fragmentation in treated cells, when compared to untreated HEp2 and HeLa cells. Thus fish venom peptide was found to selectively induce apoptosis in cancer cell.
AB - The present study is an attempt to evaluate the antiproliferative potential of peptide (7.6 kDa) from lionfish (Pterios volitans) venom on cultured HEp2 and HeLa cells. Different dose of purified peptide (1, 2 and 4 μg/ml) at different time points (12, 24 and 36 hrs) were tested for antiproliferative index of the peptide. Among them, 2 μg/ml at 24 hrs was found to effectively inhibit cancer cell growth in vitro and did not cause any adverse effect on normal human lymphocytes. Apoptosis was examined by propidium iodide staining, confirmed by the expression of caspase-8 and caspase-3, down regulation of Bcl-2 expression and DNA fragmentation in treated cells, when compared to untreated HEp2 and HeLa cells. Thus fish venom peptide was found to selectively induce apoptosis in cancer cell.
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U2 - 10.1186/1477-3163-5-27
DO - 10.1186/1477-3163-5-27
M3 - Article
C2 - 17137521
AN - SCOPUS:33845864722
SN - 0974-6773
VL - 5
JO - Journal of Carcinogenesis
JF - Journal of Carcinogenesis
M1 - 27
ER -