Functional studies of 11β-hydroxysteroid dehydrogenase

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


11β-Hydroxysteroid dehydrogenase (11-HSD) catalyzes the interconversion of cortisol and cortisone. This activity is postulated to protect the type I (mineralocorticoid) receptor from excessive concentrations of cortisol, allowing aldosterone to function as a mineralocorticoid. An enzyme with 11-HSD activity was isolated from rat liver and the corresponding rat and human cDNA and genomic clones isolated. This enzyme is a member of the "short chain dehydrogenase" family. Using site-directed mutagenesis, it was demonstrated that two highly conserved residues, Tyr-179 and Lys-183, are required for enzymatic function. Elimination of the amino terminus or the two glycosylation sites also destroys enzymatic activity. This may be due to actual disruption of enzymatic function or to effects on intracellular localization or stability of the enzyme. Examination of patients with apparent mineralocorticoid excess, a syndrome of juvenile hypertension thought to represent 11-HSD deficiency, did not reveal any mutations in the gene for this enzyme. There is substantial evidence for a second 11-HSD isozyme with distinct kinetic properties that is expressed in the renal distal tubule and possibly other sites of mineralocorticoid action. Apparent mineralocorticoid excess may involve this enzyme.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
Issue number1
StatePublished - Jan 1995


  • 11β-hydroxysteroid dehydrogenase
  • apparent mineralocorticoid excess
  • cortisol
  • cortisone
  • hypertension
  • mineralocorticoid receptor

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry


Dive into the research topics of 'Functional studies of 11β-hydroxysteroid dehydrogenase'. Together they form a unique fingerprint.

Cite this