TY - JOUR
T1 - Follicular sterol composition in gonadotrophin stimulated women with polycystic ovarian syndrome
AU - Bokal, E. Vrtačnik
AU - Tacer, K. Fon
AU - Vrbnjak, M.
AU - Lepoša, S.
AU - Virant-Klun, I.
AU - Verdenik, I.
AU - Rozman, D.
N1 - Funding Information:
The work was supported by the Ministry of Science of Slovenia, Grants J1-6713 and P1-0527. Klementina Fon Tacer was supported by the fellowship from the Ministry of Science of Slovenia. We thank M. Baltsen and A.G. Byskov for FF- and T-MAS standards.
PY - 2006/4/25
Y1 - 2006/4/25
N2 - This is the first study evaluating whether oocyte development and fertilization competence are related to intrafollicular concentration of cholesterol, meiosis-activating sterols and progesterone, after human chorionic gonadotrophin (HCG) administration of women with polycystic ovarian syndrome (PCOS). The concentration of follicular fluid meiosis-activating sterol (FF-MAS) significantly increased in the periovulatory period from 10-14 to 34-38 h after HCG administration, while the concentration of testis meiosis-activating sterol (T-MAS) decreased, suggesting a HCG-dependent inhibition of sterol Δ14-reductase. There was no correlation between follicular lanosterol, FF-MAS, T-MAS, and progesterone concentrations and the presence or absence of MII oocytes. Interestingly, free cholesterol level was significantly lower and FF-MAS/cholesterol and progesterone/cholesterol ratios significantly higher in follicles containing MII oocytes compared to follicles from which oocytes were not retrieved. Yet, fertilization and embryo quality did not correlate with follicular sterols. This knowledge should be beneficial for the implementation of protocols for in vitro maturation process, usually used in PCOS patients.
AB - This is the first study evaluating whether oocyte development and fertilization competence are related to intrafollicular concentration of cholesterol, meiosis-activating sterols and progesterone, after human chorionic gonadotrophin (HCG) administration of women with polycystic ovarian syndrome (PCOS). The concentration of follicular fluid meiosis-activating sterol (FF-MAS) significantly increased in the periovulatory period from 10-14 to 34-38 h after HCG administration, while the concentration of testis meiosis-activating sterol (T-MAS) decreased, suggesting a HCG-dependent inhibition of sterol Δ14-reductase. There was no correlation between follicular lanosterol, FF-MAS, T-MAS, and progesterone concentrations and the presence or absence of MII oocytes. Interestingly, free cholesterol level was significantly lower and FF-MAS/cholesterol and progesterone/cholesterol ratios significantly higher in follicles containing MII oocytes compared to follicles from which oocytes were not retrieved. Yet, fertilization and embryo quality did not correlate with follicular sterols. This knowledge should be beneficial for the implementation of protocols for in vitro maturation process, usually used in PCOS patients.
KW - Cholesterol
KW - Follicular fluid meiosis-activating sterol (FF-MAS)
KW - In vitro fertilization (IVF)
KW - Oocyte
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U2 - 10.1016/j.mce.2006.01.013
DO - 10.1016/j.mce.2006.01.013
M3 - Article
C2 - 16516374
AN - SCOPUS:33645930039
SN - 0303-7207
VL - 249
SP - 92
EP - 98
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 1-2
ER -