TY - JOUR
T1 - Focal dynamic thermal imaging for label-free high-resolution characterization of materials and tissue heterogeneity
AU - O’Brien, Christine M.
AU - Meng, Hongyu
AU - Shmuylovich, Leonid
AU - Carpenter, Julia
AU - Gogineni, Praneeth
AU - Zhang, Haini
AU - Bishop, Kevin
AU - Mondal, Suman B.
AU - Sudlow, Gail P.
AU - Bethea, Cheryl
AU - Bethea, Clyde
AU - Achilefu, Samuel
N1 - Funding Information:
This research was supported in part by the National Institutes of Health Grants (R01 CA171651, U54 CA199092, R01 EB021048, P50 CA094056, P30 CA091842, S10 OD020129, S10 OD016237, S10 OD027042, and S10 OD025264), the Department of Defense Breast Cancer Research Program (W81XWH-16-1-0286), and the Alvin J. Siteman Cancer Center Investment Program Research Development Award. CO was supported by a W. M. Keck Foundation Postdoctoral Research Fellowship.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - Evolution from static to dynamic label-free thermal imaging has improved bulk tissue characterization, but fails to capture subtle thermal properties in heterogeneous systems. Here, we report a label-free, high speed, and high-resolution platform technology, focal dynamic thermal imaging (FDTI), for delineating material patterns and tissue heterogeneity. Stimulation of focal regions of thermally responsive systems with a narrow beam, low power, and low cost 405 nm laser perturbs the thermal equilibrium. Capturing the dynamic response of 3D printed phantoms, ex vivo biological tissue, and in vivo mouse and rat models of cancer with a thermal camera reveals material heterogeneity and delineates diseased from healthy tissue. The intuitive and non-contact FDTI method allows for rapid interrogation of suspicious lesions and longitudinal changes in tissue heterogeneity with high-resolution and large field of view. Portable FDTI holds promise as a clinical tool for capturing subtle differences in heterogeneity between malignant, benign, and inflamed tissue.
AB - Evolution from static to dynamic label-free thermal imaging has improved bulk tissue characterization, but fails to capture subtle thermal properties in heterogeneous systems. Here, we report a label-free, high speed, and high-resolution platform technology, focal dynamic thermal imaging (FDTI), for delineating material patterns and tissue heterogeneity. Stimulation of focal regions of thermally responsive systems with a narrow beam, low power, and low cost 405 nm laser perturbs the thermal equilibrium. Capturing the dynamic response of 3D printed phantoms, ex vivo biological tissue, and in vivo mouse and rat models of cancer with a thermal camera reveals material heterogeneity and delineates diseased from healthy tissue. The intuitive and non-contact FDTI method allows for rapid interrogation of suspicious lesions and longitudinal changes in tissue heterogeneity with high-resolution and large field of view. Portable FDTI holds promise as a clinical tool for capturing subtle differences in heterogeneity between malignant, benign, and inflamed tissue.
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U2 - 10.1038/s41598-020-69362-8
DO - 10.1038/s41598-020-69362-8
M3 - Article
C2 - 32724184
AN - SCOPUS:85088649075
SN - 2045-2322
VL - 10
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 12549
ER -