TY - JOUR
T1 - Fluorescent modification of adenosine 3′,5′-monophosphate
T2 - Spectroscopic properties and activity in enzyme systems
AU - Secrist, John A.
AU - Barrio, Jorge R.
AU - Leonard, Nelson J.
AU - Villar-Palasi, Carlos
AU - Gilman, Alfred G.
PY - 1972
Y1 - 1972
N2 - The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N6-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-nonophosphate. The potential utility of this analog is indicated by its long flouorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.
AB - The synthesis of a highly fluorescent analog of adenosine 3′,5′-monophosphate, namely, 1,N6-ethenoadenosine 3′,5′-monophosphate, has provided a powerful probe for systems involving adenosine 3′,5′-nonophosphate. The potential utility of this analog is indicated by its long flouorescent lifetime, detectability at low concentration, and relatively long wavelength of excitation (300 nanometers). In protein kinase systems it is a highly acceptable substitute for adenosine 3′,5′-monophosphate.
UR - http://www.scopus.com/inward/record.url?scp=0015518517&partnerID=8YFLogxK
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U2 - 10.1126/science.177.4045.279
DO - 10.1126/science.177.4045.279
M3 - Article
C2 - 4339302
AN - SCOPUS:0015518517
SN - 0036-8075
VL - 177
SP - 279
EP - 280
JO - Science
JF - Science
IS - 4045
ER -