Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T

Henry H. Ong; Corey D. Webb; Marnie L. Gruen; Alyssa H. Hasty; John C. Gore; E. Brian Welch

doi:10.1117/12.2082333

Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T

Henry H. Ong, Corey D. Webb, Marnie L. Gruen, Alyssa H. Hasty, John C. Gore, E. Brian Welch

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Scopus citations

Abstract

Introduction: In obesity, fat-water MRI (FWMRI) methods provide valuable information about adipose tissue (AT) distribution. AT is known to undergo complex metabolic and endocrine changes in association with chronic inflammation including iron overloading. Here, we investigate the potential for FWMRI parameters (fat signal fraction (FSF), local magnetic field offset, and T₂^∗) to be sensitive to AT inflammatory changes in an established diet-induced obesity mouse model. Methods: Male C57BL/6J mice were placed on a low fat (LFD) or a high fat diet (HFD). 3D multi- gradient-echo MRI at 15.2T was performed at baseline, 4, 8, 12, and 16 weeks after diet onset. A 3D fat-water separation algorithm and additional processing was used to generate FSF, local field offset, and T₂^∗ maps. We examined these parameters in perirenal AT ROIs from HFD and LFD mice. Results: The data suggest that FSF, local field offset, and T₂^∗ can differentiate time course behavior between inflamed and control AT (increasing FSF, decreasing local field offset, increasing followed by decreasing T₂^∗). The biophysical mechanisms of these observed changes are not well understood and require further study. Conclusions: To the best of our knowledge, we report the first evidence that FWMRI can provide biomarkers sensitive to AT inflammation, and that FWMRI has the potential for longitudinal non-invasive assessment of AT inflammation in obesity.

Original language	English (US)
Title of host publication	Medical Imaging 2015
Subtitle of host publication	Biomedical Applications in Molecular, Structural, and Functional Imaging
Editors	Barjor Gimi, Robert C. Molthen
Publisher	SPIE
ISBN (Electronic)	9781628415070
DOIs	https://doi.org/10.1117/12.2082333
State	Published - 2015
Externally published	Yes
Event	Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging - Orlando, United States Duration: Feb 24 2015 → Feb 26 2015

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9417
ISSN (Print)	1605-7422

Other

Other	Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging
Country/Territory	United States
City	Orlando
Period	2/24/15 → 2/26/15

Keywords

Adipose tissue
Fat quantification
Inflammation
Iron
Mouse
MRI
Obesity

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2082333

Cite this

Ong, H. H., Webb, C. D., Gruen, M. L., Hasty, A. H., Gore, J. C., & Welch, E. B. (2015). Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T. In B. Gimi, & R. C. Molthen (Eds.), Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging Article 94170C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9417). SPIE. https://doi.org/10.1117/12.2082333

Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T. / Ong, Henry H.; Webb, Corey D.; Gruen, Marnie L. et al.
Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging. ed. / Barjor Gimi; Robert C. Molthen. SPIE, 2015. 94170C (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9417).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Ong, HH, Webb, CD, Gruen, ML, Hasty, AH, Gore, JC & Welch, EB 2015, Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T. in B Gimi & RC Molthen (eds), Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging., 94170C, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9417, SPIE, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging, Orlando, United States, 2/24/15. https://doi.org/10.1117/12.2082333

Ong HH, Webb CD, Gruen ML, Hasty AH, Gore JC, Welch EB. Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T. In Gimi B, Molthen RC, editors, Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging. SPIE. 2015. 94170C. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2082333

Ong, Henry H. ; Webb, Corey D. ; Gruen, Marnie L. et al. / Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T. Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging. editor / Barjor Gimi ; Robert C. Molthen. SPIE, 2015. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{8f232e4d5f7e472696b355e471a4820a,

title = "Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T",

abstract = "Introduction: In obesity, fat-water MRI (FWMRI) methods provide valuable information about adipose tissue (AT) distribution. AT is known to undergo complex metabolic and endocrine changes in association with chronic inflammation including iron overloading. Here, we investigate the potential for FWMRI parameters (fat signal fraction (FSF), local magnetic field offset, and T2∗) to be sensitive to AT inflammatory changes in an established diet-induced obesity mouse model. Methods: Male C57BL/6J mice were placed on a low fat (LFD) or a high fat diet (HFD). 3D multi- gradient-echo MRI at 15.2T was performed at baseline, 4, 8, 12, and 16 weeks after diet onset. A 3D fat-water separation algorithm and additional processing was used to generate FSF, local field offset, and T2∗ maps. We examined these parameters in perirenal AT ROIs from HFD and LFD mice. Results: The data suggest that FSF, local field offset, and T2∗ can differentiate time course behavior between inflamed and control AT (increasing FSF, decreasing local field offset, increasing followed by decreasing T2∗). The biophysical mechanisms of these observed changes are not well understood and require further study. Conclusions: To the best of our knowledge, we report the first evidence that FWMRI can provide biomarkers sensitive to AT inflammation, and that FWMRI has the potential for longitudinal non-invasive assessment of AT inflammation in obesity.",

keywords = "Adipose tissue, Fat quantification, Inflammation, Iron, Mouse, MRI, Obesity",

author = "Ong, {Henry H.} and Webb, {Corey D.} and Gruen, {Marnie L.} and Hasty, {Alyssa H.} and Gore, {John C.} and Welch, {E. Brian}",

note = "Publisher Copyright: {\textcopyright} 2015 SPIE.; Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging ; Conference date: 24-02-2015 Through 26-02-2015",

year = "2015",

doi = "10.1117/12.2082333",

language = "English (US)",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Barjor Gimi and Molthen, {Robert C.}",

booktitle = "Medical Imaging 2015",

}

TY - GEN

T1 - Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T

AU - Ong, Henry H.

AU - Webb, Corey D.

AU - Gruen, Marnie L.

AU - Hasty, Alyssa H.

AU - Gore, John C.

AU - Welch, E. Brian

PY - 2015

Y1 - 2015

N2 - Introduction: In obesity, fat-water MRI (FWMRI) methods provide valuable information about adipose tissue (AT) distribution. AT is known to undergo complex metabolic and endocrine changes in association with chronic inflammation including iron overloading. Here, we investigate the potential for FWMRI parameters (fat signal fraction (FSF), local magnetic field offset, and T2∗) to be sensitive to AT inflammatory changes in an established diet-induced obesity mouse model. Methods: Male C57BL/6J mice were placed on a low fat (LFD) or a high fat diet (HFD). 3D multi- gradient-echo MRI at 15.2T was performed at baseline, 4, 8, 12, and 16 weeks after diet onset. A 3D fat-water separation algorithm and additional processing was used to generate FSF, local field offset, and T2∗ maps. We examined these parameters in perirenal AT ROIs from HFD and LFD mice. Results: The data suggest that FSF, local field offset, and T2∗ can differentiate time course behavior between inflamed and control AT (increasing FSF, decreasing local field offset, increasing followed by decreasing T2∗). The biophysical mechanisms of these observed changes are not well understood and require further study. Conclusions: To the best of our knowledge, we report the first evidence that FWMRI can provide biomarkers sensitive to AT inflammation, and that FWMRI has the potential for longitudinal non-invasive assessment of AT inflammation in obesity.

AB - Introduction: In obesity, fat-water MRI (FWMRI) methods provide valuable information about adipose tissue (AT) distribution. AT is known to undergo complex metabolic and endocrine changes in association with chronic inflammation including iron overloading. Here, we investigate the potential for FWMRI parameters (fat signal fraction (FSF), local magnetic field offset, and T2∗) to be sensitive to AT inflammatory changes in an established diet-induced obesity mouse model. Methods: Male C57BL/6J mice were placed on a low fat (LFD) or a high fat diet (HFD). 3D multi- gradient-echo MRI at 15.2T was performed at baseline, 4, 8, 12, and 16 weeks after diet onset. A 3D fat-water separation algorithm and additional processing was used to generate FSF, local field offset, and T2∗ maps. We examined these parameters in perirenal AT ROIs from HFD and LFD mice. Results: The data suggest that FSF, local field offset, and T2∗ can differentiate time course behavior between inflamed and control AT (increasing FSF, decreasing local field offset, increasing followed by decreasing T2∗). The biophysical mechanisms of these observed changes are not well understood and require further study. Conclusions: To the best of our knowledge, we report the first evidence that FWMRI can provide biomarkers sensitive to AT inflammation, and that FWMRI has the potential for longitudinal non-invasive assessment of AT inflammation in obesity.

KW - Adipose tissue

KW - Fat quantification

KW - Inflammation

KW - Iron

KW - Mouse

KW - MRI

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=84943404015&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943404015&partnerID=8YFLogxK

U2 - 10.1117/12.2082333

DO - 10.1117/12.2082333

M3 - Conference contribution

AN - SCOPUS:84943404015

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Medical Imaging 2015

A2 - Gimi, Barjor

A2 - Molthen, Robert C.

PB - SPIE

T2 - Medical Imaging 2015: Biomedical Applications in Molecular, Structural, and Functional Imaging

Y2 - 24 February 2015 through 26 February 2015

ER -

Fat-water MRI is sensitive to local adipose tissue inflammatory changes in a diet-induced obesity mouse model at 15T

Abstract

Publication series

Other

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this