Facile transduction with P1 phage in Escherichia coli associated with urinary tract infections

Jacob Hogins; Sushmita Sudarshan; Philippe Zimmern; Larry Reitzer

doi:10.1016/j.mimet.2023.106722

Facile transduction with P1 phage in Escherichia coli associated with urinary tract infections

Jacob Hogins, Sushmita Sudarshan, Philippe Zimmern, Larry Reitzer

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We describe a modification of a standard method that efficiently transduces genes using P1 phage into uropathogenic Escherichia coli strains. This procedure allows utilization of the large KEIO mutant library for analysis of pathogenic E. coli strains.

Original language	English (US)
Article number	106722
Journal	Journal of Microbiological Methods
Volume	208
DOIs	https://doi.org/10.1016/j.mimet.2023.106722
State	Published - May 2023
Externally published	Yes

Keywords

Escherichia coli
Phage
Transduction
Uropathogenic

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2023.106722

Cite this

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title = "Facile transduction with P1 phage in Escherichia coli associated with urinary tract infections",

abstract = "We describe a modification of a standard method that efficiently transduces genes using P1 phage into uropathogenic Escherichia coli strains. This procedure allows utilization of the large KEIO mutant library for analysis of pathogenic E. coli strains.",

keywords = "Escherichia coli, Phage, Transduction, Uropathogenic",

author = "Jacob Hogins and Sushmita Sudarshan and Philippe Zimmern and Larry Reitzer",

note = "Funding Information: Funding was provided by a Collaborative Biomedical Research Award to LR and PZ from The University of Texas at Dallas. Publisher Copyright: {\textcopyright} 2023 Elsevier B.V.",

year = "2023",

month = may,

doi = "10.1016/j.mimet.2023.106722",

language = "English (US)",

volume = "208",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier",

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T1 - Facile transduction with P1 phage in Escherichia coli associated with urinary tract infections

AU - Hogins, Jacob

AU - Sudarshan, Sushmita

AU - Zimmern, Philippe

AU - Reitzer, Larry

PY - 2023/5

Y1 - 2023/5

N2 - We describe a modification of a standard method that efficiently transduces genes using P1 phage into uropathogenic Escherichia coli strains. This procedure allows utilization of the large KEIO mutant library for analysis of pathogenic E. coli strains.

AB - We describe a modification of a standard method that efficiently transduces genes using P1 phage into uropathogenic Escherichia coli strains. This procedure allows utilization of the large KEIO mutant library for analysis of pathogenic E. coli strains.

KW - Escherichia coli

KW - Phage

KW - Transduction

KW - Uropathogenic

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M3 - Article

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AN - SCOPUS:85152654854

SN - 0167-7012

VL - 208

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

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ER -

Facile transduction with P1 phage in Escherichia coli associated with urinary tract infections

Abstract

Keywords

ASJC Scopus subject areas

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