Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos

Chi Hun Park, Young Hee Jeoung, Kyung Jun Uh, Ki Eun Park, Jessica Bridge, Anne Powell, Jie Li, Laramie Pence, Luhui Zhang, Tianbin Liu, Hai Xi Sun, Ying Gu, Yue Shen, Jun Wu, Juan Carlos Izpisua Belmonte, Bhanu P. Telugu

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Most of our current knowledge regarding early lineage specification and embryo-derived stem cells comes from studies in rodent models. However, key gaps remain in our understanding of these developmental processes from nonrodent species. Here, we report the detailed characterization of pig extraembryonic endoderm (pXEN) cells, which can be reliably and reproducibly generated from primitive endoderm (PrE) of blastocyst. Highly expandable pXEN cells express canonical PrE markers and transcriptionally resemble rodent XENs. The pXEN cells contribute both to extraembryonic tissues including visceral yolk sac as well as embryonic gut when injected into host blastocysts, and generate live offspring when used as a nuclear donor in somatic cell nuclear transfer (SCNT). The pXEN cell lines provide a novel model for studying lineage segregation, as well as a source for genome editing in livestock.

Original languageEnglish (US)
Pages (from-to)212-223
Number of pages12
JournalStem Cell Reports
Issue number1
StatePublished - Jan 12 2021


  • Cloning
  • SCNT
  • XEN
  • embryonic chimera
  • extra-embryonic cells
  • gut endoderm
  • pig/porcine
  • primitive endoderm
  • stem cell
  • yolk sac

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Developmental Biology
  • Cell Biology


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