@article{d0b5ff40bd5e420ca0071c2121f3215e,
title = "Extraembryonic Endoderm (XEN) Cells Capable of Contributing to Embryonic Chimeras Established from Pig Embryos",
abstract = "Most of our current knowledge regarding early lineage specification and embryo-derived stem cells comes from studies in rodent models. However, key gaps remain in our understanding of these developmental processes from nonrodent species. Here, we report the detailed characterization of pig extraembryonic endoderm (pXEN) cells, which can be reliably and reproducibly generated from primitive endoderm (PrE) of blastocyst. Highly expandable pXEN cells express canonical PrE markers and transcriptionally resemble rodent XENs. The pXEN cells contribute both to extraembryonic tissues including visceral yolk sac as well as embryonic gut when injected into host blastocysts, and generate live offspring when used as a nuclear donor in somatic cell nuclear transfer (SCNT). The pXEN cell lines provide a novel model for studying lineage segregation, as well as a source for genome editing in livestock.",
keywords = "Cloning, SCNT, XEN, embryonic chimera, extra-embryonic cells, gut endoderm, pig/porcine, primitive endoderm, stem cell, yolk sac",
author = "Park, {Chi Hun} and Jeoung, {Young Hee} and Uh, {Kyung Jun} and Park, {Ki Eun} and Jessica Bridge and Anne Powell and Jie Li and Laramie Pence and Luhui Zhang and Tianbin Liu and Sun, {Hai Xi} and Ying Gu and Yue Shen and Jun Wu and {Izpisua Belmonte}, {Juan Carlos} and Telugu, {Bhanu P.}",
note = "Funding Information: This research was supported by AFRI Grants# 2015-67015-22845 and 2018-67015-27575 from the USDA National Institute of Food and Agriculture , and 1 R01 HD092304-01A1 from NICHD , NIH to B.T.; Guangdong Provincial Key Laboratory of Genome Read and Write (No. 2017B030301011), Guangdong Provincial Academician Workstation of BGI Synthetic Genomics (No. 2017B090904014), Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics (DRC-SZ[2016]884) and Shenzhen Peacock Plan (No.. KQTD20150330171505310) Funding Information: This research was supported by AFRI Grants# 2015-67015-22845 and 2018-67015-27575 from the USDA National Institute of Food and Agriculture, and 1 R01 HD092304-01A1 from NICHD, NIH to B.T.; Guangdong Provincial Key Laboratory of Genome Read and Write (No. 2017B030301011), Guangdong Provincial Academician Workstation of BGI Synthetic Genomics (No. 2017B090904014), Shenzhen Engineering Laboratory for Innovative Molecular Diagnostics (DRC-SZ[2016]884) and Shenzhen Peacock Plan (No. KQTD20150330171505310) Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2021",
month = jan,
day = "12",
doi = "10.1016/j.stemcr.2020.11.011",
language = "English (US)",
volume = "16",
pages = "212--223",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "1",
}