Abstract
The measurement of intracellular concentrations of the anti-cancer drug doxorubicin was performed by the application of a simple cell extraction technique combined with a rapid high-performance liquid chromatographic separation. Quantitation was done by fluorescence detection. The extraction procedure was non-degradative and the mean recovery of drug was 95%. A high drug extraction efficiency was confirmed with radiolabeled [3H]doxorubicin. The method is applicable to normal and neoplastic tissue.
Original language | English (US) |
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Title of host publication | Journal of Chromatography |
Pages | 139-144 |
Number of pages | 6 |
Volume | 221 |
Edition | 1 |
State | Published - 1980 |
ASJC Scopus subject areas
- Clinical Biochemistry
- Molecular Medicine