@article{f88bad3c66e04c68a9bf3eb8874a791b,
title = "Extended Lifetime in Vivo Pulse Stimulated Ultrasound Imaging",
abstract = "An on-demand long-lived ultrasound contrast agent that can be activated with single pulse stimulated imaging (SPSI) has been developed using hard shell liquid perfluoropentane filled silica 500-nm nanoparticles for tumor ultrasound imaging. SPSI was tested on LnCAP prostate tumor models in mice; tumor localization was observed after intravenous (IV) injection of the contrast agent. Consistent with enhanced permeability and retention, the silica nanoparticles displayed an extended imaging lifetime of 3.3±1 days (mean±standard deviation). With added tumor specific folate functionalization, the useful lifetime was extended to 12 ± 2 days; in contrast to ligand-based tumor targeting, the effect of the ligands in this application is enhanced nanoparticle retention by the tumor. This paper demonstrates for the first time that IV injected functionalized silica contrast agents can be imaged with an in vivo lifetime 500 times longer than current microbubble-based contrast agents. Such functionalized long-lived contrast agents may lead to new applications in tumor monitoring and therapy.",
keywords = "Contrast agents, nanoparticles, prostate, ultrasound, visualization",
author = "James Wang and Barback, {Christopher V.} and Ta, {Casey N.} and Joi Weeks and Natalie Gude and Mattrey, {Robert F.} and Blair, {Sarah L.} and Trogler, {William C.} and Hotaik Lee and Kummel, {Andrew C.}",
note = "Funding Information: The authors declare the following competing financial interest(s): A.C.K. and W.C.T., scientific cofounders, have an equity interest in Nanocyte Medical, Inc., a company that may potentially benefit from the research results, and serve on the companys Scientific Advisory Board. S.L.B. has a family member with an equity interest in Nanocyte Medical, Inc., a company that may potentially benefit from the research results. The terms of this arrangement have been reviewed and approved by the University of California, San Diego, in accordance with its conflict of interest policies. This work was supported by National Institute of Health NIH under Grant 1R33CA177449, Grant NIH-NCINCTC-R21T, and Grant 1F31CA177199, in part by the T32 Training Grant, and in part by the Samsung Advanced Institute of Technology. Funding Information: Manuscript received June 15, 2017; revised August 5, 2017; accepted August 7, 2017. Date of publication August 17, 2017; date of current version December 29, 2017. This work was supported by National Institute of Health NIH under Grant 1R33CA177449, Grant NIH-NCI-NCTC-R21T, and Grant 1F31CA177199, in part by the T32 Training Grant, and in part by the Samsung Advanced Institute of Technology. (Corresponding author: James Wang.) J. Wang is with the Department of Nanoengineering, University of California at San Diego, San Diego, CA 92093 USA (e-mail: jaw059@ucsd.edu). Publisher Copyright: {\textcopyright} 2017 IEEE.",
year = "2018",
month = jan,
doi = "10.1109/TMI.2017.2740784",
language = "English (US)",
volume = "37",
pages = "222--229",
journal = "IEEE Transactions on Medical Imaging",
issn = "0278-0062",
publisher = "Institute of Electrical and Electronics Engineers Inc.",
number = "1",
}