TY - JOUR
T1 - Expression proteomics identifies biochemical adaptations and defense responses in transgenic plants with perturbed polyamine metabolism
AU - Franceschetti, Marina
AU - Perry, Barry
AU - Thompson, Benjamin
AU - Hanfrey, Colin
AU - Michael, Anthony J.
N1 - Funding Information:
M.F. was supported by an E.U. Marie Curie fellowship (MCFI 2000-02029). We thank Julie Hofer for constructive criticism of the manuscript.
PY - 2004/10/22
Y1 - 2004/10/22
N2 - Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.
AB - Soluble proteins from leaves of transgenic tobacco plants with perturbed polyamine metabolism, caused by S-adenosylmethionine decarboxylase overexpression, were analysed by comparative proteomics. A group of proteins was found to be increasingly repressed, in parallel with the degree of polyamine perturbation, in each of the three independent transgenic lines. These were identified as isoforms of chloroplast ribonucleoproteins, known to be involved in chloroplast mRNA stability, processing and translation. Another group of eight proteins strongly induced in the most metabolically perturbed line was identified as multiple, uncharacterised isoforms of the defense protein PR-1, a known marker for systemic acquired resistance.
KW - Chloroplast ribonucleoprotein
KW - PR-1
KW - Polyamine
KW - Proteomic
KW - S-Adenosylmethionine decarboxylase
UR - http://www.scopus.com/inward/record.url?scp=6344223618&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=6344223618&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2004.08.085
DO - 10.1016/j.febslet.2004.08.085
M3 - Article
C2 - 15498583
AN - SCOPUS:6344223618
SN - 0014-5793
VL - 576
SP - 477
EP - 480
JO - FEBS Letters
JF - FEBS Letters
IS - 3
ER -