TY - JOUR
T1 - Expression of β-1,4-galactosyltransferase I in CD4+ T H cells and its effect on cellular adhesion ability
AU - Han, Yu
AU - Wang, Xiao Ying
AU - Ji, Yu Hong
AU - Shen, Ai Guo
AU - Sun, Xiao Lei
AU - Hu, Ying Qing
AU - Zhou, Xiao Rang
AU - Wu, Qiong
PY - 2007/10/31
Y1 - 2007/10/31
N2 - Objective: To study the expression and localization of β-1,4- galactosyltransferase I (β-1,4-GalT I) in CD4+ helper T(T H)lymphocytes and the effect of β-1,4-GalT I on adhesion ability of CD4+ TH cells. Methods: CD4+ TH cells were isolated by CD4 positive immunomagnetic beads. The expression and localization of β-1,4-GalT I in CD4+ TH cells was detected by RT-PCR, immunofluorescence and laser scanning confocal microscopy. Activation and differentiation of CD4+ TH cells were introduced by cytokines such as rhIL-2, rhIL-12 and rhIL-4. The expression level of β-1,4-GalT I in CD4+ TH cells was measured by semi-quantitative RT-PCR and flow cytometry. The adhesion ability of CD4 + TH cells was compared by cell adhesion assay. α-lactalbumin was used to interfere the activity and substrate specificity of cell surface β-1,4-GalT I, then the influence of that on the adhesion ability of CD4+ TH cells was analyzed by cell adhesion assay. Results: Long and short β-1,4-GalT I were expressed and localized on the cytomembrane and cytoplasm of CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I, and the adhesion ability of CD4+ TH cells activated with rhIL-2 increased as compared with unactivated CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I were both positively correlated with the adhesion ability of CD4+ TH cells. The adhesion ability of CD4+ TH cells decreased after the incubation with α-lactalbumin. Conclusion: β-1,4-GalT I expression in CD4+ TH cells is closely correlated with cell adhesion ability, which suggests that β-1,4-GalT I may play an important role in the adhesion process of CD4 + TH cells.
AB - Objective: To study the expression and localization of β-1,4- galactosyltransferase I (β-1,4-GalT I) in CD4+ helper T(T H)lymphocytes and the effect of β-1,4-GalT I on adhesion ability of CD4+ TH cells. Methods: CD4+ TH cells were isolated by CD4 positive immunomagnetic beads. The expression and localization of β-1,4-GalT I in CD4+ TH cells was detected by RT-PCR, immunofluorescence and laser scanning confocal microscopy. Activation and differentiation of CD4+ TH cells were introduced by cytokines such as rhIL-2, rhIL-12 and rhIL-4. The expression level of β-1,4-GalT I in CD4+ TH cells was measured by semi-quantitative RT-PCR and flow cytometry. The adhesion ability of CD4 + TH cells was compared by cell adhesion assay. α-lactalbumin was used to interfere the activity and substrate specificity of cell surface β-1,4-GalT I, then the influence of that on the adhesion ability of CD4+ TH cells was analyzed by cell adhesion assay. Results: Long and short β-1,4-GalT I were expressed and localized on the cytomembrane and cytoplasm of CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I, and the adhesion ability of CD4+ TH cells activated with rhIL-2 increased as compared with unactivated CD4+ TH cells. The expression level of long β-1,4-GalT I and cell surface β-1,4-GalT I were both positively correlated with the adhesion ability of CD4+ TH cells. The adhesion ability of CD4+ TH cells decreased after the incubation with α-lactalbumin. Conclusion: β-1,4-GalT I expression in CD4+ TH cells is closely correlated with cell adhesion ability, which suggests that β-1,4-GalT I may play an important role in the adhesion process of CD4 + TH cells.
KW - CD4 helper T lymphocytes
KW - Cellular adhesion
KW - β-1,4-galactosyltransferase I
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M3 - Article
AN - SCOPUS:45149118032
SN - 0254-5101
VL - 27
SP - 871
EP - 877
JO - Chinese Journal of Microbiology and Immunology
JF - Chinese Journal of Microbiology and Immunology
IS - 10
ER -