TY - JOUR
T1 - Expression of β-1,4-galactosyltransferase-I affects cellular adhesion in human peripheral blood CD4+ T cells
AU - Han, Yu
AU - Zhou, Xiaorong
AU - Ji, Yuhong
AU - Shen, Aiguo
AU - Sun, Xiaolei
AU - Hu, Yingqing
AU - Wu, Qiong
AU - Wang, Xiaoying
N1 - Funding Information:
This work was supported by National Natural Scientific Grants ( 30300099 , 30800998 ), PR China and the Nantong Technology Foundation ( K2007022 ).
PY - 2010
Y1 - 2010
N2 - β-1,4-galactosyltransferase-I (β-1,4-GalT-I) has two isoforms that differ only in the length of their cytoplasmic domains. In this study, we found that both the long and short isoforms of β-1,4-GalT-I were expressed in human CD4+ T lymphocytes, and localized in the cytoplasm and on the plasma membrane. The expression level of β-1,4-GalT-I was increased in CD4+ T cells after stimulation with interleukin (IL)-2, and was further increased after stimulation with IL-2 + IL-12, but decreased after stimulation with IL-2 + IL-4 when compared to stimulation with IL-2 alone. We also demonstrated that the cellular adhesion of CD4+ T cells was significantly increased upon cytokine stimulation, and was inhibited by α-lactalbumin, indicating that the increase in adhesion was positively correlated with the expression and activity of long β-1,4-GalT-I. Collectively, the data suggest that β-1,4-GalT-I plays a role in the cellular adhesion of CD4+ T cells.
AB - β-1,4-galactosyltransferase-I (β-1,4-GalT-I) has two isoforms that differ only in the length of their cytoplasmic domains. In this study, we found that both the long and short isoforms of β-1,4-GalT-I were expressed in human CD4+ T lymphocytes, and localized in the cytoplasm and on the plasma membrane. The expression level of β-1,4-GalT-I was increased in CD4+ T cells after stimulation with interleukin (IL)-2, and was further increased after stimulation with IL-2 + IL-12, but decreased after stimulation with IL-2 + IL-4 when compared to stimulation with IL-2 alone. We also demonstrated that the cellular adhesion of CD4+ T cells was significantly increased upon cytokine stimulation, and was inhibited by α-lactalbumin, indicating that the increase in adhesion was positively correlated with the expression and activity of long β-1,4-GalT-I. Collectively, the data suggest that β-1,4-GalT-I plays a role in the cellular adhesion of CD4+ T cells.
KW - CD4 helper T lymphocytes
KW - Cellular adhesion
KW - β-1,4-galactosyltransferase-I
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U2 - 10.1016/j.cellimm.2009.08.004
DO - 10.1016/j.cellimm.2009.08.004
M3 - Article
C2 - 20202494
AN - SCOPUS:77349124718
SN - 0008-8749
VL - 262
SP - 11
EP - 17
JO - Cellular Immunology
JF - Cellular Immunology
IS - 1
ER -