Expression of ricin A chain in Escherichia coli

Mary O'Hare, Lynne M. Roberts, Philip E. Thorpe, Graham J. Watson, Bernadette Prior, J. Michael Lord

Research output: Contribution to journalArticlepeer-review

76 Scopus citations


DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalFEBS Letters
Issue number1
StatePublished - May 25 1987


  • (E.coli)
  • Expression
  • Immunotoxin
  • Ricin A chain

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology


Dive into the research topics of 'Expression of ricin A chain in Escherichia coli'. Together they form a unique fingerprint.

Cite this