Expression of ricin A chain in Escherichia coli

Mary O'Hare; Lynne M. Roberts; Philip E. Thorpe; Graham J. Watson; Bernadette Prior; J. Michael Lord

doi:10.1016/0014-5793(87)80759-7

Expression of ricin A chain in Escherichia coli

Mary O'Hare, Lynne M. Roberts, Philip E. Thorpe, Graham J. Watson, Bernadette Prior, J. Michael Lord

Pharmacology

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter P_N25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

Original language	English (US)
Pages (from-to)	73-78
Number of pages	6
Journal	FEBS Letters
Volume	216
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(87)80759-7
State	Published - May 25 1987

Keywords

(E.coli)
Expression
Immunotoxin
Ricin A chain

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(87)80759-7

Cite this

@article{54ad81029e8842a59a5c844b26148c7d,

title = "Expression of ricin A chain in Escherichia coli",

abstract = "DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.",

keywords = "(E.coli), Expression, Immunotoxin, Ricin A chain",

author = "Mary O'Hare and Roberts, {Lynne M.} and Thorpe, {Philip E.} and Watson, {Graham J.} and Bernadette Prior and Lord, {J. Michael}",

note = "Funding Information: This work was supported by the Engineering Research Council GR/D/8 1770.",

year = "1987",

month = may,

day = "25",

doi = "10.1016/0014-5793(87)80759-7",

language = "English (US)",

volume = "216",

pages = "73--78",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Expression of ricin A chain in Escherichia coli

AU - O'Hare, Mary

AU - Roberts, Lynne M.

AU - Thorpe, Philip E.

AU - Watson, Graham J.

AU - Prior, Bernadette

AU - Lord, J. Michael

N1 - Funding Information: This work was supported by the Engineering Research Council GR/D/8 1770.

PY - 1987/5/25

Y1 - 1987/5/25

N2 - DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

AB - DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter PN25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.

KW - (E.coli)

KW - Expression

KW - Immunotoxin

KW - Ricin A chain

UR - http://www.scopus.com/inward/record.url?scp=0023664423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023664423&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(87)80759-7

DO - 10.1016/0014-5793(87)80759-7

M3 - Article

C2 - 3556218

AN - SCOPUS:0023664423

SN - 0014-5793

VL - 216

SP - 73

EP - 78

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Expression of ricin A chain in Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this