Abstract
We investigated the effect of constitutive active Akt expression on anti-proliferative and apoptotic effect of tamoxifen in MCF-7 human breast cancer cells. Forced expression of AktDD (T308D, S473D) resulted in increased phosphorylation of GSK3β, a physiological substrate of Akt. When estrogen receptor (ER) mediated transcription was determined by luciferase assays, there was more than 2-fold increase in estradiol-dependent transcription in MCF-7 cells overexpressing AktDD (MCF-7 AktDD) compared to vector control cells (MCF-7 vec). MCF-7 AktDD cells showed increased proliferation in a medium containing charcoal stripped serum supplemented with estradiol. When the cell cycle profiles were examined, there was an increase in S-phase and a reduction in G1 phase in MCF-7 AktDD cells as compared to MCF-7 vec cells. Overexpression of AktDD also attenuated tamoxifen-mediated apoptosis. These results suggest that Akt could confer resistance to anti-estrogen mediated cell death and inhibition of proliferation.
Original language | English (US) |
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Pages (from-to) | 664-669 |
Number of pages | 6 |
Journal | IUBMB life |
Volume | 58 |
Issue number | 11 |
DOIs | |
State | Published - Nov 1 2006 |
Keywords
- Akt signaling
- Breast cancer cells
- Tamoxifen
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Genetics
- Clinical Biochemistry
- Cell Biology