Expression in lung cancer of a transcribed sequence at the DNF15S2 locus at chromosome 3P21

J. W. Harbour; S. L. Lai; A. F. Gazdar; J. D. Minna; F. J. Kaye

Expression in lung cancer of a transcribed sequence at the DNF15S2 locus at chromosome 3P21

J. W. Harbour, S. L. Lai, A. F. Gazdar, J. D. Minna, F. J. Kaye

Research output: Contribution to journal › Article › peer-review

Abstract

The short arm of chromosome 3 undergoes genetic loss in virtually all small cell lung cancers (SCLC). The most frequently deleted region includes the DNF15S2 locus (mapped to 3p21), suggesting that a putative recessive SCLC gene might be located nearby. Using a DNA probe subcloned from the DNF15S2 locus, were identified a 3.0 kb mRNA species and isolated corresponding partial cDNA clones. Analysis of the nucleic acid sequence showed it to be the same as a recently published transcribed gene isolated from this chromosomal 3p21 region. To determine whether this gene might represent a 'recessive oncogene' we examined a large number of SCLC and other human tumors and found no evidence for DNA rearrangements or inactivation of RNA expression. We discuss the significance of this transcribed gene and the DNF15S2 locus in tumorigenesis.

Original language	English (US)
Pages (from-to)	23-27
Number of pages	5
Journal	Anticancer Research
Volume	10
Issue number	1
State	Published - 1990

Keywords

chromosome 3
restriction fragment length polymorphism
small cell carcinoma

ASJC Scopus subject areas

Oncology
Cancer Research

Cite this

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title = "Expression in lung cancer of a transcribed sequence at the DNF15S2 locus at chromosome 3P21",

abstract = "The short arm of chromosome 3 undergoes genetic loss in virtually all small cell lung cancers (SCLC). The most frequently deleted region includes the DNF15S2 locus (mapped to 3p21), suggesting that a putative recessive SCLC gene might be located nearby. Using a DNA probe subcloned from the DNF15S2 locus, were identified a 3.0 kb mRNA species and isolated corresponding partial cDNA clones. Analysis of the nucleic acid sequence showed it to be the same as a recently published transcribed gene isolated from this chromosomal 3p21 region. To determine whether this gene might represent a 'recessive oncogene' we examined a large number of SCLC and other human tumors and found no evidence for DNA rearrangements or inactivation of RNA expression. We discuss the significance of this transcribed gene and the DNF15S2 locus in tumorigenesis.",

keywords = "chromosome 3, restriction fragment length polymorphism, small cell carcinoma",

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year = "1990",

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TY - JOUR

T1 - Expression in lung cancer of a transcribed sequence at the DNF15S2 locus at chromosome 3P21

AU - Harbour, J. W.

AU - Lai, S. L.

AU - Gazdar, A. F.

AU - Minna, J. D.

AU - Kaye, F. J.

PY - 1990

Y1 - 1990

N2 - The short arm of chromosome 3 undergoes genetic loss in virtually all small cell lung cancers (SCLC). The most frequently deleted region includes the DNF15S2 locus (mapped to 3p21), suggesting that a putative recessive SCLC gene might be located nearby. Using a DNA probe subcloned from the DNF15S2 locus, were identified a 3.0 kb mRNA species and isolated corresponding partial cDNA clones. Analysis of the nucleic acid sequence showed it to be the same as a recently published transcribed gene isolated from this chromosomal 3p21 region. To determine whether this gene might represent a 'recessive oncogene' we examined a large number of SCLC and other human tumors and found no evidence for DNA rearrangements or inactivation of RNA expression. We discuss the significance of this transcribed gene and the DNF15S2 locus in tumorigenesis.

AB - The short arm of chromosome 3 undergoes genetic loss in virtually all small cell lung cancers (SCLC). The most frequently deleted region includes the DNF15S2 locus (mapped to 3p21), suggesting that a putative recessive SCLC gene might be located nearby. Using a DNA probe subcloned from the DNF15S2 locus, were identified a 3.0 kb mRNA species and isolated corresponding partial cDNA clones. Analysis of the nucleic acid sequence showed it to be the same as a recently published transcribed gene isolated from this chromosomal 3p21 region. To determine whether this gene might represent a 'recessive oncogene' we examined a large number of SCLC and other human tumors and found no evidence for DNA rearrangements or inactivation of RNA expression. We discuss the significance of this transcribed gene and the DNF15S2 locus in tumorigenesis.

KW - chromosome 3

KW - restriction fragment length polymorphism

KW - small cell carcinoma

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Expression in lung cancer of a transcribed sequence at the DNF15S2 locus at chromosome 3P21

Abstract

Keywords

ASJC Scopus subject areas

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