Expression and function of transplantation antigens with altered or deleted cytoplasmic domains

Martha C. Zuniga, Bernard Malissen, Minnie McMillan, Peter R. Brayton, Stephen S. Clark, James Forman, Leroy Hood

Research output: Contribution to journalArticlepeer-review

67 Scopus citations


Two mutants of the class I gene encoding the H-2Ld transplantation antigen have been constructed. In one mutant the cytoplasmic domain of the class I molecule has been altered by deletion of 24 of the 31 C-terminal residues, and in the second the C-terminal 25 residues of the cytoplasmic domain have been replaced with a unique sequence of 19 amino acids. These mutant class I genes have been transferred into mouse L cells by DNA-mediated gene transfer. Both mutant genes are expressed at normal levels on the cell surface, and they have charge properties and sizes consistent with the introduced alterations. These mutant Ld molecules can serve as target antigens for allogeneic cytotoxic T cells and as restricting elements for virus-specific cytotoxic T cells. These results show that the 24 residues replaced or deleted from the carboxy terminus of the class I molecule are not required for its transport to or integration in the plasma membrane, nor for its function as a target antigen or a restricting element during T-cell-mediated cytotoxicity.

Original languageEnglish (US)
Pages (from-to)535-544
Number of pages10
Issue number2
StatePublished - Sep 1983

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


Dive into the research topics of 'Expression and function of transplantation antigens with altered or deleted cytoplasmic domains'. Together they form a unique fingerprint.

Cite this