TY - JOUR
T1 - Evaluation of saliva as a source of human DNA for population and association studies
AU - Quinque, Dominique
AU - Kittler, Ralf
AU - Kayser, Manfred
AU - Stoneking, Mark
AU - Nasidze, Ivan
PY - 2006/6/15
Y1 - 2006/6/15
N2 - A simple noninvasive procedure for saliva sample collection and DNA extraction was developed. On average, the amount of human DNA (as measured by a TaqMan-based assay) was about 11.4 μg/mL saliva, which is more than can be obtained from other noninvasive samples such as cheek swabs. However, the presence of large amounts of nonhuman DNA (up to 90% of the total extracted DNA) in saliva samples does necessitate DNA quantitation methods that are specific for human DNA. We were able to reliably and accurately type different genetic markers (mDNA sequences, Y-chromosomal single-nucleotide polymorphisms, and autosomal microsatellite loci) from saliva samples stored for up to 30 days at 37 °C, making this method well-suited for field conditions and convenient transportation of samples back to the laboratory. Thus, saliva can be considered a reliable source of DNA for a wide variety of genetic studies.
AB - A simple noninvasive procedure for saliva sample collection and DNA extraction was developed. On average, the amount of human DNA (as measured by a TaqMan-based assay) was about 11.4 μg/mL saliva, which is more than can be obtained from other noninvasive samples such as cheek swabs. However, the presence of large amounts of nonhuman DNA (up to 90% of the total extracted DNA) in saliva samples does necessitate DNA quantitation methods that are specific for human DNA. We were able to reliably and accurately type different genetic markers (mDNA sequences, Y-chromosomal single-nucleotide polymorphisms, and autosomal microsatellite loci) from saliva samples stored for up to 30 days at 37 °C, making this method well-suited for field conditions and convenient transportation of samples back to the laboratory. Thus, saliva can be considered a reliable source of DNA for a wide variety of genetic studies.
KW - DNA extraction method
KW - Saliva
KW - Sample collection
UR - http://www.scopus.com/inward/record.url?scp=33646832209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646832209&partnerID=8YFLogxK
U2 - 10.1016/j.ab.2006.03.021
DO - 10.1016/j.ab.2006.03.021
M3 - Article
C2 - 16620753
AN - SCOPUS:33646832209
SN - 0003-2697
VL - 353
SP - 272
EP - 277
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -