Original language | English (US) |
---|---|
Pages (from-to) | 2927-2930 |
Number of pages | 4 |
Journal | Cell Cycle |
Volume | 5 |
Issue number | 24 |
DOIs | |
State | Published - Dec 15 2006 |
Keywords
- AcCoA
- DUF738
- Fold assignment
- Function prediction
- N-acetyltransferase
ASJC Scopus subject areas
- Molecular Biology
- Developmental Biology
- Cell Biology
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In: Cell Cycle, Vol. 5, No. 24, 15.12.2006, p. 2927-2930.
Research output: Contribution to journal › Letter › peer-review
}
TY - JOUR
T1 - Eukaryotic domain of unknown function DUF738 belongs to Gcn5-related N-acetyltransferase superfamily
AU - Steczkiewicz, Kamil
AU - Kinch, Lisa
AU - Grishin, Nick V.
AU - Rychlewski, Leszek
AU - Ginalski, Knysztof
N1 - Funding Information: Exhaustive, transitive PSI-BLAST7 searches (E-value threshold 0.01) against the NCBI nonredundant protein database initiated with DUF738 consensus sequence detected in total 54 uncharacterized protein sequences entirely from eukaryotic organisms including mammals, flies, bony fishes, ciliates, amphibians, echinoderms, beetles, nematodes, flatworms and kinetoplastids. Further searches with standard sequence comparison tools such as CDD8 and SMART9 using collected DUF738 family members did not yield any significant hits to known protein domains. However, application of meta profile alignment method Meta-BASIC,10 that uses comparison of sequence profiles This work was supported in part by the NIH enriched by predicted secondary structure, resulted in confident assignment of DUF738 grant GM67165 and the Welch Foundation proteins to GNAT superfamily. Specifically, Meta-BASIC mapped DUF738 consensus Grant I-1505 to N.V.G., and by MNI and sequence with an above threshold scores (>12) of 15.6 to Saccharomyces cerevisiae Esa1 the European Commission with grants histone acetyltransferase3 (pdb|1fy7) and 14.1 to Pfam family MOZ/SAS (accession LSHG-CT-2003-503265 and LSHB-CT- number: PF01853), which has been suggested to be homologous to acetyltransferases.11 2003-503017 to L.R. and by the MNiSW Meta-BASIC prediction was further confirmed by consensus fold recognition 3D-Jury grant PBZ-MNiI-2/1/2005 to K.G. server12 that provided consistent matches (with reliable confidence scores13 above 50) to variety of AcCoA N-acetyltransferase structures including Bacillus subtilis acetyltransferase PaiA14 (pdb|1tiq), Saccharomyces cerevisiae histone acetyltransferase Hpa215 (pdb|1qsm), Salmonella enteritidis aminoglycoside N-acetyltransferase AAC(6’)-Iy16 (pdb|1s3z) and ©2006 LANDES BIOSCIENCE transcriptional regulator from Bacillus halodurans (pdb|1z4e). Conservation of GNAT superfamily motifs and a reasonably good mapping of predicted and observed secondary structures are additional indicators of the correct assignment.
PY - 2006/12/15
Y1 - 2006/12/15
KW - AcCoA
KW - DUF738
KW - Fold assignment
KW - Function prediction
KW - N-acetyltransferase
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U2 - 10.4161/cc.5.24.3572
DO - 10.4161/cc.5.24.3572
M3 - Letter
C2 - 17172875
AN - SCOPUS:33845666508
SN - 1538-4101
VL - 5
SP - 2927
EP - 2930
JO - Cell Cycle
JF - Cell Cycle
IS - 24
ER -